MYO18B Antibody | MyBioSource MBS9217052 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MYO18B Antibody

catalog :

MBS9217052

quantity :

0.05 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1683CT270.3950]

reactivity :

human

application :

western blot, ELISA, flow cytometry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9217052 staining MYO18B in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

image 2 :

MBS9217052 staining MYO18B in human heart tissue sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. Aundiluted biotinylated goat polyvalent antibodywas used as the secondary antibody

image 3 :

Overlay histogram showing A431 cells stainedwith MBS9217052(green line). The cells were fixedwith 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by theantibody (AM8558b, 1:25 dilution) for 60 min at37ºC. The secondary antibody used wasGoat-Anti-Mouse IgG, DyLight® 488Conjugated Highly Cross-Adsorbed(OJ192088)at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b(1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed

product information

catalog number :

MBS9217052

products type :

Antibody

products full name :

MYO18B Antibody

products short name :

[MYO18B]

products name syn :

[Unconventional myosin-XVIIIb; MYO18B]

other names :

[unconventional myosin-XVIIIb isoform 1; Unconventional myosin-XVIIIb; unconventional myosin-XVIIIb; myosin XVIIIB]

products gene name :

[MYO18B]

other gene names :

[MYO18B; MYO18B; KFS4]

uniprot entry name :

MY18B_HUMAN

clonality :

Monoclonal

isotype :

IgG2b,k

clone :

[1683CT270.3950]

host :

Mouse

reactivity :

Human

sequence length :

2568

specificity :

This MYO18B antibody is generated from a mouse immunized with recombinant protein from human MYO18B.

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC), Western Blot (WB), ELISA (EIA)

app notes :

Immunohistochemistry-Paraffin ~~1:25. Flow Cytometry~~1:25. Western Blot (WB)~~1:6000

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Testing Data (TD)

image4 heading :

Testing Data (TD)

image4 description :

Anti-MYO18B Antibody at 1:16000 dilution +Recombinant protein Lysates/proteins at 20 ?gper lane. Secondary Goat Anti-Mouse IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 285kDa Blocking/Dilution buffer: 5% NFDM/TBST

other info1 :

Calculated MW: 285215. Subtitle: Purified Mouse Monoclonal Antibody (Mab). Cellular Location: Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere. Note=Punctate pattern in undifferentiated myoblasts Nuclear, on primary cardiomyocytes and adult muscle. A partial sarcomeric location was found in some cardiomyocytes.

other info2 :

Tissue Location: Selectively expressed in cardiac and skeletal muscles. Weakly expressed in testis, pancreas, placenta, prostate, lung and thymus. Antigen Type: Recombinant Protein

products categories :

New Antibodies; Primary Antibodies; Signal Transduction

products description :

May be involved in intracellular trafficking of the muscle cell when in the cytoplasm, whereas entering the nucleus, may be involved in the regulation of muscle specific genes. May play a role in the control of tumor development and progression; restored MYO18B expression in lung cancer cells suppresses anchorage-independent growth.

products references :

Nishioka M.,et al.Proc. Natl. Acad. Sci. U.S.A. 99:12269-12274 (2002). Salamon M.,et al.J. Mol. Biol. 326:137-149 (2003). Gu Y.,et al.Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases. Dunham I.,et al.Nature 402:489-495 (1999). Stanchi F.,et al.Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.

ncbi gi num :

970949420

ncbi acc num :

NP_001305174.1

ncbi gb acc num :

NM_001318245.1

uniprot acc num :

Q8IUG5

ncbi summary :

The protein encoded by this gene may regulate muscle-specific genes when in the nucleus and may influence intracellular trafficking when in the cytoplasm. The encoded protein functions as a homodimer and may interact with F actin. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jul 2008]

uniprot summary :

MYO18B: May be involved in intracellular trafficking of the muscle cell when in the cytoplasm, whereas entering the nucleus, may be involved in the regulation of muscle specific genes. May play a role in the control of tumor development and progression; restored MYO18B expression in lung cancer cells suppresses anchorage-independent growth. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motor. Chromosomal Location of Human Ortholog: 22q12.1. Cellular Component: nucleus; sarcomere; unconventional myosin complex. Molecular Function: actin binding; ATP binding; motor activity. Biological Process: metabolic process. Disease: Klippel-feil Syndrome 4, Autosomal Recessive, With Myopathy And Facial Dysmorphism

size1 :

0.05 mL

price1 :

165 USD

size2 :

0.2 mL

price2 :

370

more info or order :

MyBioSource product webpage