CD81 Antibody | MyBioSource MBS9217050 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD81 Antibody

catalog :

MBS9217050

quantity :

0.05 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes:. Anti-CD81 Antibody at 1:2000 dilution . Lane 1: A549 whole cell lysate . Lane 2: Ramos whole cell lysate . Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa . Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Anti-CD81 Antibody at 1:4000 dilution + human testis whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

Anti-CD81 Antibody at 1:4000 dilution + rat cerebellum whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9217050

products type :

Antibody

products full name :

CD81 Antibody

products short name :

[CD81]

products name syn :

[CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD81; CD81; TAPA1; TSPAN28]

other names :

[CD81 antigen isoform 2; CD81 antigen; CD81 antigen; CD81 molecule; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD_antigen: CD81]

products gene name :

[CD81]

products gene name syn :

[TAPA1; TSPAN28]

other gene names :

[CD81; CD81; S5.7; CVID6; TAPA1; TSPAN28; TAPA1; TSPAN28; Tspan-28]

uniprot entry name :

CD81_HUMAN

clonality :

Monoclonal

isotype :

IgG1,k

host :

Mouse

reactivity :

Human

sequence length :

165

specificity :

This CD81 antibody is generated from a mouse immunized with recombinant protein from the human region of human CD81.

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB:~~1:4000

image1 heading :

Test Data

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

other info1 :

Subtitle: Purified Mouse Monoclonal Antibody (Mab). Cellular Location: Membrane; Multi-pass membrane protein. Clone Names: 1668CT270.93.9

other info2 :

Tissue Location: Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

products categories :

Crown Antibodies; New Antibodies; Primary Antibodies; Immunology; Microbiology

products description :

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.

products references :

Oren R.,et al.Mol. Cell. Biol. 10:4007-4015 (1990). Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Livingston R.J.,et al.Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases. Taylor T.D.,et al.Nature 440:497-500 (2006). Takahashi S.,et al.J. Immunol. 145:2207-2213 (1990).

ncbi gi num :

663071018

ncbi acc num :

NP_001284578.1

ncbi gb acc num :

NM_001297649.1

uniprot acc num :

P60033

ncbi mol weight :

25809

ncbi pathways :

Adaptive Immune System Pathway (366160); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Malaria Pathway (152665); Malaria Pathway (152657)

ncbi summary :

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

uniprot summary :

CD81: May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6); also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the tetraspanin (TM4SF) family. Protein type: Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: apical plasma membrane; focal adhesion; immunological synapse; integral to plasma membrane; membrane; plasma membrane; vesicle. Molecular Function: protein binding. Biological Process: activation of MAPK activity; cell proliferation; cell surface receptor linked signal transduction; entry of virus into host cell; positive regulation of 1-phosphatidylinositol 4-kinase activity; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein localization; receptor internalization; regulation of growth; regulation of immune response; regulation of protein stability; response to wounding; virion attachment, binding of host cell surface receptor. Disease: Immunodeficiency, Common Variable, 6

size1 :

0.05 mL

price1 :

165 USD

size2 :

0.2 mL

price2 :

370

more info or order :

MyBioSource product webpage