ISG15 Antibody (N-term) | MyBioSource MBS9217047 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ISG15 Antibody (N-term)

catalog :

MBS9217047

quantity :

0.05 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1031CT2.8.3]

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-ISG15 Antibody (N-term)) at 1:8000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9217047

products type :

Antibody

products full name :

ISG15 Antibody (N-term)

products short name :

[ISG15]

products name syn :

[Ubiquitin-like protein ISG15; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP; ISG15; G1P2; UCRP]

other names :

[ubiquitin-like protein ISG15; Ubiquitin-like protein ISG15; ubiquitin-like protein ISG15; ISG15 ubiquitin-like modifier; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP]

products gene name :

[ISG15]

products gene name syn :

[G1P2; UCRP]

other gene names :

[ISG15; ISG15; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP; G1P2; UCRP; IP17; hUCRP]

uniprot entry name :

ISG15_HUMAN

clonality :

Monoclonal

isotype :

IgG1,k

clone :

[1031CT2.8.3]

host :

Mouse

reactivity :

Human

sequence length :

165

specificity :

This ISG15 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-165 amino acids from the N-terminal region of human ISG15.

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB:~~1:8000

image1 heading :

Western Blot (WB)

other info1 :

Cellular Location: Cytoplasm. Secreted. Note=Exists in three distinct states: free within the cell, released into the extracellular space, or conjugated to target proteins.

other info2 :

Tissue Location: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Expressed in neutrophils, monocytes and lymphocytes. Enhanced expression seen in pancreatic adenocarcinoma, endometrial cancer, and bladder cancer, as compared to non-cancerous tissue. In bladder cancer, the increase in expression exhibits a striking positive correlation with more advanced stages of the disease.

products categories :

Crown Antibodies; New Antibodies; Primary Antibodies; Cell Biology

products description :

Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Can also isgylate: EIF2AK2/PKR which results in its activation, DDX58/RIG-I which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation- inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade therby inhibiting IFNA- induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity.

products references :

Blomstrom D.C.,et al.J. Biol. Chem. 261:8811-8816 (1986). Reich N.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:6394-6398 (1987). Knight E. Jr.,et al.J. Biol. Chem. 263:4520-4522 (1988). Knight E. Jr.,et al.J. Biol. Chem. 263:10040-10040 (1988). Kamitani T.,et al.Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.

ncbi gi num :

4826774

ncbi acc num :

NP_005092.1

ncbi gb acc num :

NM_005101.3

uniprot acc num :

P05161

ncbi mol weight :

17888 Da

ncbi pathways :

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Innate Immune System Pathway (106387); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (187102); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128)

ncbi summary :

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]

uniprot summary :

ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Protein type: Ubiquitin-like modifier. Chromosomal Location of Human Ortholog: 1p36.33. Cellular Component: cytosol; extracellular region; nucleoplasm. Molecular Function: protein binding; protein tag. Biological Process: bypass DNA synthesis; defense response to bacterium; defense response to virus; ISG15-protein conjugation; modification-dependent protein catabolic process; negative regulation of interferon type I production; negative regulation of protein ubiquitination; negative regulation of viral genome replication; positive regulation of erythrocyte differentiation; regulation of interferon-gamma production; viral reproduction. Disease: Immunodeficiency 38

size1 :

0.05 mL

price1 :

165 USD

size2 :

0.2 mL

price2 :

370

more info or order :

MyBioSource product webpage