TWF1 Antibody (Center) | MyBioSource MBS9216710 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TWF1 Antibody (Center)

catalog :

MBS9216710

quantity :

0.05 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-TWF1 Antibody (Center) at 1:2000 dilution. Lane 1: NCI-H1299 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: mouse brain lysate. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9216710

products type :

Antibody

products full name :

TWF1 Antibody (Center)

products short name :

[TWF1]

products name syn :

[Twinfilin-1, Protein A6, Protein tyrosine kinase 9, TWF1, PTK9]

other names :

[twinfilin-1 isoform 1; Twinfilin-1; twinfilin-1; twinfilin actin binding protein 1; Protein A6; Protein tyrosine kinase 9]

products gene name :

[TWF1]

other gene names :

[TWF1; TWF1; A6; PTK9; PTK9]

uniprot entry name :

TWF1_HUMAN

clonality :

Polyclonal

isotype :

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

357

specificity :

This TWF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 201-235 amino acids from the Central region of human TWF1.

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB)

app notes :

WB ~~ 1:2000

image1 heading :

Western Blot (WB)

other info2 :

Function: Actin-binding protein involved in motile andmorphological processes. Inhibits actinpolymerization, likely by sequesteringG-actin. By capping the barbed ends offilaments, it also regulates motility. Seems to play an important role in clathrin-mediate dendocytosis and distribution of endocytic organelles (By similarity). Cellular location: Cellular Location Cytoplasm. Cytoplasm, cytoskeleton.Note=Diffuse cytoplasmic localization withper inuclear and G-actin-rich cortical actinstructures sublocalization. Also found at membrane ruffles and cell-cell contacts (Bysimilarity). Tissue location: Tissue Location Expressed at high levels in the colon, testis,ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Notdetected in liver.

products categories :

Signal Transduction

products description :

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).

products references :

Beeler J.F.,et al.Mol. Cell. Biol. 14:982-988(1994). Ota T.,et al.Nat. Genet. 36:40-45(2004). Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.

ncbi gi num :

334848198

ncbi acc num :

NP_001229326.1

ncbi gb acc num :

NM_001242397.1

uniprot acc num :

Q12792

ncbi mol weight :

40283

ncbi summary :

This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]

uniprot summary :

A6: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down- regulated. Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 12q12. Cellular Component: focal adhesion; myofibril; perinuclear region of cytoplasm; cytoplasm; intercellular junction; filopodium; actin cytoskeleton. Molecular Function: actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein-tyrosine kinase activity; ATP binding. Biological Process: negative regulation of actin filament polymerization; sequestering of actin monomers; regulation of actin phosphorylation

size1 :

0.05 mL

price1 :

165 USD

size2 :

0.2 mL

price2 :

370

more info or order :

MyBioSource product webpage