FEV Antibody (N-Term) | MyBioSource MBS9215640 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FEV Antibody (N-Term)

catalog :

MBS9215640

quantity :

0.05 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9215640 staining FEV in human smallintestine sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated withprimary antibody (1/25) for 1 hours at 37°C. Aundiluted biotinylated goat polyvalent antibodywas used as the secondary antibody.

image 2 :

Anti-FEV Antibody (N-Term) at 1:2000 dilution+ K562 whole cell lysate Lysates/proteins at 20?g per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9215640

products type :

Antibody

products full name :

FEV Antibody (N-Term)

products short name :

[FEV]

products name syn :

[Purified Rabbit Polyclonal Antibody (Pab); Protein FEV; Fifth Ewing variant protein; PC12 ETS domain-containing transcription factor 1; PC12 ETS factor 1; Pet-1; FEV; PET1]

other names :

[protein FEV; Protein FEV; protein FEV; FEV (ETS oncogene family); Fifth Ewing variant protein; PC12 ETS domain-containing transcription factor 1; PC12 ETS factor 1; Pet-1]

products gene name :

[FEV]

other gene names :

[FEV; FEV; PET-1; HSRNAFEV; PET1; PC12 ETS factor 1; Pet-1]

uniprot entry name :

FEV_HUMAN

clonality :

Polyclonal

isotype :

host :

Rabbit

reactivity :

Human

sequence length :

238

specificity :

This FEV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 17-49 amino acids from human FEV.

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunohistochemistry -Paraffin, (IHC-P), Western Blot(WB), ELISA (EIA)

app notes :

IHC-P~~1:25. WB~~1:2000

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Testing Data (TD)

other info1 :

Calculated MW: 25030

other info2 :

Function: Functions as a transcriptional regulator.According to PubMed: 12761502 , it functionsas a transcriptional repressor. Functions inthe differentiation and the maintenance ofthe central serotonergic neurons. May play arole in cell growth.Cellular Location: Nucleus {ECO:0000255 PROSITE-ProRule:PRU00237,ECO:0000269 PubMed:12761502}. Tissue Location: In brain, exclusively expressed in the majorserotonergic neurons of the dorsal andmedian raphe nuclei located in the midbrainand pons. Also detected in prostate and small intestine.

products categories :

Crown Antibodies; Neuroscience

products description :

Functions as a transcriptional regulator. According to PubMed: 12761502 , it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.

products references :

Peter M.,et al.Oncogene 14:1159-1164(1997). Hillier L.W.,et al.Nature 434:724-731(2005). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Maurer P.,et al.Oncogene 22:3319-3329(2003). Maurer P.,et al.Neurosci. Lett. 357:215-218(2004).

ncbi gi num :

8923789

ncbi acc num :

NP_059991.1

ncbi gb acc num :

NM_017521.2

uniprot acc num :

Q99581

ncbi pathways :

SIDS Susceptibility Pathways (198901); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

This gene belongs to the ETS transcription factor family. ETS family members have a highly conserved 85-amino acid ETS domain that binds purine-rich DNA sequences. The alanine-rich C-terminus of this gene indicates that it may act as a transcription repressor. This gene is exclusively expressed in neurons of the central serotonin (5-HT) system, a system implicated in the pathogeny of such psychiatric diseases as depression, anxiety, and eating disorders. In some types of Ewing tumors, this gene is fused to the Ewing sarcoma (EWS) gene following chromosome translocations. [provided by RefSeq, Jul 2008]

uniprot summary :

FEV: Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth. Genetic variation in FEV may be associated with susceptibility to sudden infant death syndrome (SIDS). SIDS remains elusive in its causes and devastating in its consequences. Despite the impressive decline in the incidence of SIDS since the recommendation to avoid the prone sleep position, SIDS remains a leading cause of death in the first year of life. A chromosomal aberration involving FEV is found in Ewing tumors. Translocation t(2;21;22)(q23;q22;q12) that forms a EWSR1-FEV fusion protein with a potential oncogenic activity. Belongs to the ETS family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 2q36. Cellular Component: nucleus. Molecular Function: sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity; transcription corepressor activity. Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; neuron fate specification; cell differentiation; neuron maturation

size1 :

0.05 mL

price1 :

165 USD

size2 :

0.2 mL

price2 :

370

more info or order :

MyBioSource product webpage