GAPDH Antibody, HRP Conjugate | MyBioSource MBS9215532 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GAPDH Antibody, HRP Conjugate

catalog :

MBS9215532

quantity :

0.05 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

[1A10A10]

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-GAPDH Antibody, HRP Conjugate at 1:40000 dilution + Hela whole cell lysate. Lysates/proteins at 20 ug per lane. Predicted band size : 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9215532

products type :

Antibody

products full name :

GAPDH Antibody, HRP Conjugate

products short name :

[GAPDH]

products name syn :

[Purified Mouse Monoclonal Antibody (Mab); GAPD]

other names :

[glyceraldehyde-3-phosphate dehydrogenase isoform 2; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)]

products gene name :

[GAPDH]

other gene names :

[GAPDH; GAPDH; G3PD; GAPD; HEL-S-162eP; GAPD; GAPDH]

uniprot entry name :

G3P_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[1A10A10]

host :

Mouse

reactivity :

Human

sequence length :

293

specificity :

This GAPDH antibody is generated from a mouse immunized with a recombinant protein of human GAPDH.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), Elisa (EIA)

app notes :

WB ~~ 1:40000

image1 heading :

Western Blot (WB)

other info1 :

Antigen Source: Human

other info2 :

Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities,thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis.Nuclear functions are probably due to thenitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Cellular Location: Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity).Postnuclear and Perinuclear regions.

products categories :

Stem Cells; Signal Transduction; Neuroscience; Microbiology; Metabolism; Cancer; Antibody Collections; Primary Antibodies; New Antibodies; Crown Antibodies; Loading Control

ncbi gi num :

378404908

ncbi acc num :

NP_001243728.1

ncbi gb acc num :

NM_001256799.2

uniprot acc num :

P04406

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Antibiotics Pathway (1144997); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Gluconeogenesis Pathway (1269961); Glucose Metabolism Pathway (1269959)

ncbi summary :

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

uniprot summary :

GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma. Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; EC 1.2.1.12. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: microtubule cytoskeleton; nuclear membrane; intracellular membrane-bound organelle; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; lipid particle; ribonucleoprotein complex; nucleus; cytosol; vesicle. Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding. Biological Process: neuron apoptosis; protein stabilization; negative regulation of translation; carbohydrate metabolic process; glucose metabolic process; microtubule cytoskeleton organization and biogenesis; gluconeogenesis

size1 :

0.05 mL

price1 :

165 USD

size2 :

0.2 mL

price2 :

370

more info or order :

MyBioSource product webpage