RAB3B Antibody | MyBioSource MBS9215444 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAB3B Antibody

catalog :

MBS9215444

quantity :

0.05 mL

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1543CT354.60.92]

reactivity :

human, mouse, rat

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Overlay histogram showing HepG2 cells stained with MBS9215444 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9215444, 1:25 dilution) for 60 min at 37 C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(at 1/400 dilution for 40 min at 37 C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image 2 :

staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

image 3 :

All lanes : Anti-RAB3B Antibody at 1:2000 dilution. Lane 1: mouse brain lysate. Lane 2: rat brain lysate. Lane 3: SW480 whole cell lysate. Lane 4: human brain lysate. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9215444

products type :

Antibody

products full name :

RAB3B Antibody

products short name :

[RAB3B]

products name syn :

[Purified Mouse Monoclonal Antibody (Mab); Ras-related protein Rab-3B; RAB3B]

other names :

[ras-related protein Rab-3B; Ras-related protein Rab-3B; ras-related protein Rab-3B; RAB3B, member RAS oncogene family]

products gene name :

[RAB3B]

other gene names :

[RAB3B; RAB3B]

uniprot entry name :

RAB3B_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

[1543CT354.60.92]

host :

Mouse

reactivity :

Human, Mouse, Rat

sequence length :

219

specificity :

This RAB3B antibody is generated from a mouse immunized with a recombinant protein of human RAB3B.

form :

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA, Flow Cytometry (FC), Immunohistochemistry -Paraffin (IHC-P)

app notes :

FC~~1:25. IHC-P~~1:25. WB~~1:2000

image1 heading :

Flow Cytometry

image2 heading :

Immunohistochemistry-Paraffin

image3 heading :

Western Blot (WB)

other info1 :

Antigen Source: Human

other info2 :

Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side

products categories :

Crown Antibodies; Cancer; Neuroscience; Signal Transduction

products description :

Protein transport. Probably involved in vesicular traffic (By similarity).

products references :

Zahraoui A.,et al.J. Biol. Chem. 264:12394-12401(1989). Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases. Gregory S.G.,et al.Nature 441:315-321(2006). Rigbolt K.T.,et al.Sci. Signal. 4:RS3-RS3(2011).

ncbi gi num :

19923750

ncbi acc num :

NP_002858.2

ncbi gb acc num :

NM_002867.3

uniprot acc num :

P20337

ncbi mol weight :

24758

ncbi pathways :

Tight Junction Pathway (83071); Tight Junction Pathway (482)

ncbi summary :

RAB3B: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family. Protein type: G protein; G protein, monomeric; G protein, monomeric, Rab. Chromosomal Location of Human Ortholog: 1p32-p31. Cellular Component: synaptic vesicle; perinuclear region of cytoplasm; secretory granule membrane; cytoplasm; plasma membrane; cytosol; endosome; vesicle. Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; myosin V binding. Biological Process: regulation of exocytosis; antigen processing and presentation; intracellular protein transport; synaptic vesicle exocytosis; positive regulation of dopamine uptake; protein secretion; peptidyl-cysteine methylation; Rab protein signal transduction; vesicle docking during exocytosis

uniprot summary :

RAB3B: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family. Protein type: G protein; G protein, monomeric; G protein, monomeric, Rab. Chromosomal Location of Human Ortholog: 1p32-p31. Cellular Component: cytoplasm; cytosol; perinuclear region of cytoplasm; plasma membrane; secretory granule; synaptic vesicle; vesicle. Molecular Function: GDP binding; GTP binding; GTPase activity; myosin V binding; protein binding. Biological Process: antigen processing and presentation; peptidyl-cysteine methylation; positive regulation of dopamine uptake; protein transport; regulation of exocytosis; small GTPase mediated signal transduction

size1 :

0.05 mL

price1 :

165 USD

size2 :

0.2 mL

price2 :

370

more info or order :

MyBioSource product webpage