catalog number :
MBS921527
products type :
ELISA Kit
products full name :
Pig lactate dehydrogenase, LDH ELISA Kit
products short name :
lactate dehydrogenase (LDH)
products name syn :
Pig lactate dehydrogenase (LDH) ELISA Kit; lactate dehydrogenase (LDH)
other names :
lactate dehydrogenase; lactate dehydrogenase
specificity :
This assay has high sensitivity and excellent specificity for detection of pig LDH. No significant cross-reactivity or interference between pig LDH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 125 ?U /ml -8000 ?U/ml. Sensitivity: The minimum detectable dose of pig LDH is typically less than 62.5 ?U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest pig LDH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LDH. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LDH. The competitive inhibition reaction is launched between with pre-coated LDH and LDH in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LDH in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001095933.1
ncbi gb acc num :
NM_001102463.1
ncbi pathways :
Cysteine And Methionine Metabolism Pathway (872115); Cysteine And Methionine Metabolism Pathway (103421); Glycolysis / Gluconeogenesis Pathway (872099); Glycolysis / Gluconeogenesis Pathway (287); Metabolic Pathways (872183); Propanoate Metabolism Pathway (872157); Propanoate Metabolism Pathway (387); Pyruvate Metabolism Pathway (872144); Pyruvate Metabolism Pathway (373)
size4 :
10x96-Strip-Wells