CLU Antibody (N-term) | MyBioSource MBS9215265 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CLU Antibody (N-term)

catalog :

MBS9215265

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysates from A549 cell line and human plasma tissue lysate(from left to right), using CLU Antibody (N-term). MBS9215265 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

image 2 :

Western blot analysis of lysates from Hela cell line, human liver and human testis tissue lysate(from left to right), using CLU Antibody (N-term). MBS9215265 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

image 3 :

CLU antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLU antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9215265

products type :

Antibody

products full name :

CLU Antibody (N-term)

products short name :

[CLU]

products name syn :

[Clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Apo-J; Complement cytolysis inhibitor; CLI; Complement-associated protein SP-40; Ku70-binding protein 1; NA1/NA2; Testosterone-repressed prostate message 2; TRPM-2; Clusterin beta chain; ApoJalpha; Complement cytolysis inhibitor a chain; Clusterin alpha chain; ApoJbeta; Complement cytolysis inhibitor b chain; CLU; APOJ; CLI; KUB1]

other names :

[clusterin preproprotein; Clusterin; clusterin; clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Apo-J]

products gene name :

[CLU]

other gene names :

[CLU; CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; APOJ; CLI; KUB1; Apo-J; CLI; TRPM-2]

uniprot entry name :

CLUS_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse, rat

sequence length :

460

specificity :

This CLU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-99 amino acids from the N-terminal region of human CLU.

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

FC, WB, IHC-P, E

app notes :

FC~~1:25. WB~~1:500-1000. IHC-P~~1:50~100

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

Overlay histogram showing Hela cells stained with MBS9125265 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9215265, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image5 heading :

Testing Data TD

image5 description :

All lanes : Anti-CLU Antibody (N-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: Caco2 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: A549 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

other info1 :

. Antigen Region: 71-99. Cellular location: Isoform 1: Secreted. Note=Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Tissue Location: Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung .

products categories :

Cancer; Cardiovascular; Cell Biology; Immunology; Neuroscience; Signal Transduction

products description :

The protein encoded by this gene appears to be involved in. several basic biological events such as cell death, tumor. progression, and neurodegenerative disorders. However, the function. of this protein is unknown. Three transcript variants encoding. different isoforms have been found for this gene, and one of them. is secreted and processed into a mature form.

products references :

Jun, G., et al. Arch. Neurol. (2010) In press :. Zhou, Y., et al. DNA Cell Biol. (2010) In press :. Mengel-From, J., et al. Neurobiol. Aging (2010) In press :. Golenkina, S.A., et al. Mol. Biol. (Mosk.) 44(4):620-626(2010). Thambisetty, M., et al. Arch. Gen. Psychiatry 67(7):739-748(2010)

ncbi gi num :

355594753

ncbi acc num :

NP_001164609.1

ncbi gb acc num :

NM_001831.3

uniprot acc num :

P10909

ncbi pathways :

Pantothenate And CoA Biosynthesis Pathway (1191); Pantothenate And CoA Biosynthesis Pathway (1191); Pantothenate And CoA Biosynthesis Pathway (1191); Pantothenate And CoA Biosynthesis Pathway (1191); Pantothenate And CoA Biosynthesis Pathway (1191)

ncbi summary :

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]

uniprot summary :

CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Mitochondrial; Apoptosis; Secreted. Chromosomal Location of Human Ortholog: 8p21-p12. Cellular Component: extracellular matrix; Golgi apparatus; extracellular space; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; mitochondrial membrane; cytoplasm; extracellular region; cytosol; nucleus. Molecular Function: protein binding; ubiquitin protein ligase binding; chaperone binding; ATPase activity; misfolded protein binding. Biological Process: platelet activation; response to misfolded protein; release of cytochrome c from mitochondria; positive regulation of nitric oxide biosynthetic process; protein stabilization; positive regulation of apoptosis; response to virus; cell morphogenesis; microglial cell activation; positive regulation of tumor necrosis factor production; complement activation; activation of NF-kappaB transcription factor; negative regulation of protein homooligomerization; reverse cholesterol transport; platelet degranulation; myelin maintenance in the central nervous system; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein import; innate immune response; lipid metabolic process; chaperone-mediated protein complex assembly; blood coagulation; complement activation, classical pathway

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage