MYBPC3 Antibody (N-term) | MyBioSource MBS9215190 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MYBPC3 Antibody (N-term)

catalog :

MBS9215190

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[RB31023]

reactivity :

human, mouse, rat

application :

western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9215190 staining MYBPC3 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

image 2 :

MBS9215190staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

image 3 :

MBS9215190 staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

product information

catalog number :

MBS9215190

products type :

Antibody

products full name :

MYBPC3 Antibody (N-term)

products short name :

[MYBPC3]

products name syn :

[Myosin-binding protein C; cardiac-type; Cardiac MyBP-C; C-protein; cardiac muscle isoform; MYBPC3]

other names :

[myosin-binding protein C, cardiac-type; Myosin-binding protein C, cardiac-type; myosin-binding protein C, cardiac-type; myosin binding protein C, cardiac; C-protein, cardiac muscle isoform]

products gene name :

[MYBPC3]

other gene names :

[MYBPC3; MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C; Cardiac MyBP-C]

uniprot entry name :

MYPC3_HUMAN

clonality :

Polyclonal

isotype :

Rabbit IgG

clone :

[RB31023]

host :

Rabbit

reactivity :

Human, Mouse, Rat(Predicted Reactivity: Rat)

sequence positions :

[189-218]

sequence length :

1,273

specificity :

This MYBPC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the N-terminal region of human MYBPC3.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

app notes :

IHC-P~~1:25. WB~~1:2000

image1 heading :

Immunohistochemistry

image2 heading :

Immunohistochemistry

image3 heading :

Immunohistochemistry

image4 heading :

Western Blot

image4 description :

All lanes : Anti-MYBPC3 Antibody (N-term) at 1:2000 dilution Lane 1: mouse heart lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 141 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image5 heading :

Western Blot

image5 description :

All lanes : Anti-MYBPC3 Antibody (N-term) at 1:1000-1:2000 dilution Lane 1: human liver lysate Lane 2: mouse heart lysate Lane 3: rat heart lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 141 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

other info2 :

Function: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

products categories :

Cardiovascular

products description :

MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy.

products references :

Millat, G., et al. Clin. Chim. Acta 411 (23-24), 1983-1991 (2010) :. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Millat, G., et al. Eur J Med Genet 53(5):261-267(2010). Zimmerman, R.S., et al. Genet. Med. 12(5):268-278(2010). Brion, M., et al. Ann. Clin. Lab. Sci. 40(3):285-289(2010)

ncbi gi num :

148596957

ncbi acc num :

NP_000247.2

ncbi gb acc num :

NM_000256.3

uniprot acc num :

Q14896

ncbi pathways :

Porphyrin And Chlorophyll Metabolism Pathway (4607); Porphyrin And Chlorophyll Metabolism Pathway (4607); Porphyrin And Chlorophyll Metabolism Pathway (4607); Porphyrin And Chlorophyll Metabolism Pathway (4607); Porphyrin And Chlorophyll Metabolism Pathway (4607); Porphyrin And Chlorophyll Metabolism Pathway (4607); Porphyrin And Chlorophyll Metabolism Pathway (4607)

ncbi summary :

uniprot summary :

MYBPC3: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Belongs to the immunoglobulin superfamily. MyBP family. Protein type: Myosin-binding. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: sarcomere; striated muscle thick filament; cytosol; A band. Molecular Function: identical protein binding; ATPase activator activity; myosin binding; structural constituent of muscle; metal ion binding; titin binding; actin binding; myosin heavy chain binding. Biological Process: heart morphogenesis; regulation of heart rate; positive regulation of ATPase activity; regulation of muscle filament sliding; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; myosin filament assembly; cell adhesion; cardiac muscle contraction; muscle filament sliding. Disease: Left Ventricular Noncompaction 10; Cardiomyopathy, Familial Hypertrophic, 4

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage