Mouse Protein Wnt-10b, WNT10B ELISA Kit | MyBioSource MBS921518 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Protein Wnt-10b, WNT10B ELISA Kit

catalog :

MBS921518

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS921518

products type :

ELISA Kit

products full name :

Mouse Protein Wnt-10b, WNT10B ELISA Kit

products short name :

wingless-type MMTV integration site family, member 10B

products name syn :

Mouse Protein Wnt-10b (WNT10B) ELISA kit; SHFM6; WNT-12; WNT-10B protein; wingless-type MMTV integration site family; member 10B

other names :

protein Wnt-10b; Protein Wnt-10b; protein Wnt-10b; wnt-12; wingless related MMTV integration site 10b; wingless-type MMTV integration site family, member 10B; Protein Wnt-12

products gene name :

WNT10B

other gene names :

Wnt10b; Wnt10b; Wnt12; Wnt-10b; Wnt10; Wnt12

uniprot entry name :

WN10B_MOUSE

reactivity :

Mouse

sequence length :

389

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse WNT10B. No significant cross-reactivity or interference between mouse WNT10B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 9.4 pg/ml -600 pg/ml. Sensitivity: 2.35 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT10B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT10B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT10B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT10B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6756003

ncbi acc num :

NP_035848.1

ncbi gb acc num :

NM_011718.2

uniprot acc num :

P48614

ncbi mol weight :

43,119 Da

ncbi pathways :

Adipogenesis Pathway (198299); Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); Developmental Biology Pathway (575210); ESC Pluripotency Pathways (198374); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83289)

uniprot summary :

WNT10B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6). SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. Belongs to the Wnt family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: frizzled binding; receptor binding. Biological Process: regulation of fat cell differentiation; positive regulation of apoptosis; multicellular organismal development; regulation of protein metabolic process; skeletal muscle fiber development; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; signal transduction; neuron differentiation; cell-cell signaling; positive regulation of cell proliferation; positive regulation of epithelial cell differentiation; G2/M transition of mitotic cell cycle; positive regulation of transcriptional preinitiation complex assembly; cell cycle arrest; negative regulation of epithelial cell proliferation; smoothened signaling pathway; Wnt receptor signaling pathway; cell fate commitment; sensory perception of taste; protein stabilization; regulation of proteasomal ubiquitin-dependent protein catabolic process; skeletal muscle regeneration; negative regulation of fat cell differentiation; positive regulation of bone mineralization; positive regulation of ossification; regulation of transcription from RNA polymerase II promoter; positive regulation of osteoblast differentiation; organ morphogenesis; regulation of skeletal muscle development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; lipid metabolic process; positive regulation of anagen; myoblast cell differentiation involved in skeletal muscle regeneration

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage