CF150 Antibody (Center) | MyBioSource MBS9214999 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CF150 Antibody (Center)

catalog :

MBS9214999

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Flow cytometric analysis of A549 cells using CF150 Antibody (Center) (green) compared to an isotype control of rabbit IgG(blue). MBS9214999 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

image 2 :

Western blot analysis of lysate from THP-1 cell line, using CF150 Antibody (Center). MBS9214999 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

image 3 :

Western blot analysis of lysates from MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate(from left to right), using CF150 Antibody (Center). MBS9214999 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

product information

catalog number :

MBS9214999

products type :

Antibody

products full name :

CF150 Antibody (Center)

products short name :

[CF150]

products name syn :

[Cyclic GMP-AMP synthase; cGAMP synthase; cGAS; h-cGAS; Mab-21 domain-containing protein 1; MB21D1; C6orf150]

other names :

[cyclic GMP-AMP synthase; Cyclic GMP-AMP synthase; cyclic GMP-AMP synthase; Mab-21 domain containing 1; Mab-21 domain-containing protein 1]

products gene name :

[MB21D1]

other gene names :

[MB21D1; MB21D1; cGAS; h-cGAS; C6orf150; C6orf150; cGAMP synthase; cGAS; h-cGAS]

uniprot entry name :

CGAS_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[266-295]

sequence length :

447

specificity :

This CF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human CF150.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)

app notes :

FC~~1:10~50. WB~~1:500-1:1000

image1 heading :

Flow Cytometry (FC/FACS)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of lysates from HT-29, THP-1 cell line, human cerebellum tissue lysate (from left to right), using CF150 Antibody (Center). MBS9214999 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

image5 heading :

Flow Cytometry (FC/FACS)

image5 description :

CF150 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Cellular Location: Cytoplasm, cytosol Note=Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1)(PubMed:26048138)

products categories :

Cell Biology

products description :

The exact function of C6orf150 remains unknown.

products references :

Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Mungall, A.J., et al. Nature 425(6960):805-811(2003)

ncbi gi num :

115511030

ncbi acc num :

NP_612450.2

ncbi gb acc num :

NM_138441.2

uniprot acc num :

Q8N884

ncbi mol weight :

58814

ncbi pathways :

Glycerolipid Metabolism Pathway (115004); Glycerolipid Metabolism Pathway (115004); Glycerolipid Metabolism Pathway (115004); Glycerolipid Metabolism Pathway (115004); Glycerolipid Metabolism Pathway (115004); Glycerolipid Metabolism Pathway (115004)

uniprot summary :

MB21D1: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2 ,5 phosphodiester linkage at the GpA step and the formation of a 3 ,5 phosphodiester linkage at the ApG step, producing c[G(2 ,5 )pA(3 ,5 )p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. Protein type: Enzyme, cellular metabolism; EC 2.7.7.86. Chromosomal Location of Human Ortholog: 6q13. Cellular Component: cytosol. Molecular Function: GTP binding; DNA binding; metal ion binding; ATP binding. Biological Process: cyclic nucleotide biosynthetic process; positive regulation of interferon type I production; innate immune response; positive regulation of defense response to virus by host; activation of innate immune response; defense response to virus

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage