COLEC11 Antibody (N-term) | MyBioSource MBS9214722 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

COLEC11 Antibody (N-term)

catalog :

MBS9214722

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of COLEC11 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). COLEC11 (arrow) was detected using the purified Pab.

image 2 :

Formalin-fixed and paraffin-embedded human hepatocarcinoma with COLEC11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 3 :

Flow cytometric analysis of MDA-231 cells using COLEC11 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9214722

products type :

Antibody

products full name :

COLEC11 Antibody (N-term)

products short name :

[COLEC11]

products name syn :

[Collectin-11; Collectin kidney protein 1; CL-K1; COLEC11]

other names :

[collectin-11 isoform c; Collectin-11; collectin-11; collectin sub-family member 11; Collectin kidney protein 1; CL-K1]

products gene name :

[COLEC11]

other gene names :

[COLEC11; COLEC11; 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb; CL-K1]

uniprot entry name :

COL11_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[50-78]

sequence length :

285

specificity :

This COLEC11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the N-terminal region of human COLEC11.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

concentration :

Vial Concentration: 2

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

app notes :

WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of COLEC11 (arrow) using rabbit polyclonal COLEC11 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COLEC11 gene.

other info1 :

Antigen Type: Synthetic Peptide. Clone Names: RB19578

other info2 :

Antigen Source: Human. Function: Lectin that plays a role in innate immunity, apoptosis and embryogenesis (PubMed: 23954398 , PubMed: 25912189 , PubMed: 21258343 ). Calcium-dependent lectin that binds self and non-self glycoproteins presenting high mannose oligosaccharides with at least one terminal alpha-1,2-linked mannose epitope (PubMed: 25912189 ). Primarily recognizes the terminal disaccharide of the glycan (PubMed: 25912189 ). Also recognizes a subset of fucosylated glycans and lipopolysaccharides (PubMed: 17179669 , PubMed: 25912189 ). Plays a role in innate immunity through its ability to bind non-self sugars presented by microorganisms and to activate the complement through the recruitment of MAPS1 (PubMed: 20956340 , PubMed: 25912189 ). Also plays a role in apoptosis through its ability to bind in a calcium-independent manner the DNA present at the surface of apoptotic cells and to activate the complement in response to this binding (Probable). Finally, plays a role in development, probably serving as a guidance cue during the migration of neural crest cells and other cell types during embryogenesis (PubMed: 21258343 , PubMed: 28301481 ). Cellular Location: Secreted. Tissue Location: Ubiquitous (PubMed:17179669). Detected in adrenal gland, kidney, liver, ovaries and testis (at protein level) (PubMed:20956340).

products categories :

Cell Biology

products description :

COLEC11 is a member of the collectin family of C-type lectins, which contain a collagen-like domain and a carbohydrate recognition domain, and play a role in host-defense

products references :

Oguri,M., et.al., Am. J. Hypertens. (2009)

ncbi gi num :

364502941

ncbi acc num :

NP_001242911.1

ncbi gb acc num :

NM_001255982.1

uniprot acc num :

Q9BWP8

ncbi mol weight :

28665

ncbi summary :

This gene encodes a member of the collectin family of C-type lectins that possess collagen-like sequences and carbohydrate recognition domains. Collectins are secreted proteins that play important roles in the innate immune system by binding to carbohydrate antigens on microorganisms, facilitating their recognition and removal. The encoded protein binds to multiple sugars with a preference for fucose and mannose. Mutations in this gene are a cause of 3MC syndrome-2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

COLEC11: Lectin that binds to various sugars including fucose and mannose. Has a higher affinity for fucose compared to mannose. Does not bind to glucose, N-acetylglucosamine and N- acetylgalactosamine. Also binds lipopolysaccharides (LPS). Involved in fundamental development serving as a guidance cue for neural crest cell migration. Defects in COLEC11 are the cause of 3MC syndrome type 2 (3MC2). 3MC2 is an a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly archedDE eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes. Belongs to the COLEC10/COLEC11 family. 9 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 2p25.3. Cellular Component: collagen; extracellular region. Molecular Function: mannose binding. Biological Process: receptor-mediated endocytosis; multicellular organismal development; developmental process. Disease: 3mc Syndrome 2

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage