TRPM7 (CHAK1) Antibody (C-term) | MyBioSource MBS9214600 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TRPM7 (CHAK1) Antibody (C-term)

catalog :

MBS9214600

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of anti-CHAK1 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). CHAK1 (arrow) was detected using the purified Pab.

image 2 :

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

image 3 :

CHAK1 Antibody (F1835) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).

product information

catalog number :

MBS9214600

products type :

Antibody

products full name :

TRPM7 (CHAK1) Antibody (C-term)

products short name :

[TRPM7 (CHAK1)]

products name syn :

[CHAK1, LTRPC7]

other names :

[transient receptor potential cation channel subfamily M member 7 isoform a; Transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel, subfamily M, member 7; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7]

products gene name :

[TRPM7]

other gene names :

[TRPM7; TRPM7; CHAK; CHAK1; ALSPDC; LTRPC7; LTrpC-7; TRP-PLIK; CHAK1; LTRPC7; LTrpC-7; LTrpC7]

uniprot entry name :

TRPM7_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, Mouse; Predicted: Rat

sequence positions :

[1821-1850]

sequence length :

1,865

specificity :

This TRPM7 (CHAK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1821-1850 amino acids from the C-terminal region of human TRPM7 (CHAK1).

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Parrafin (IHC-P)

app notes :

WB~~1:1000. IHC-P~~1:50~100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Western Blot (WB)

other info1 :

Function: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF- induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1). Cellular Location: Membrane; Multi-pass membrane protein

products categories :

Neuroscience

products description :

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.[supplied by OMIM]

products references :

Blume-Jensen P, et al. Nature 2001. 411: 355. Cantrell D, J. Cell Sci. 2001. 114: 1439. Jhiang S Oncogene 2000. 19: 5590. Manning G, et al. Science 2002. 298: 1912. Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359. Robertson, S. et al. Trends Genet. 2000. 16: 368. Robinson D, et al. Oncogene 2000. 19: 5548. Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889. Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561. Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

ncbi gi num :

148612863

ncbi acc num :

NP_060142.3

ncbi gb acc num :

NM_017672.5

uniprot acc num :

Q96QT4

ncbi summary :

The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014]

uniprot summary :

ChaK1: is an unusual and ubiquitous bifunctional protein containing a transient receptor potential (TRP) ion channel fused to a protein kinase of the Atypical alpha group. Required for early embryonic development and for maintaining cardiac automaticity. The channel conducts Zn(2+), Mg(2+), and Ca(2+) as well as monovalent cations. The kinase can be cleaved from the channel domain. The cleaved kinase translocates to the nucleus where it binds chromatin-remodeling complexes including Polycomb, and phosphorylates specific histone residues. The kinase activity is essential for the channel function, and channel function is critical to maintain physiological intracellular magnesium levels. Plays a key role in oxidative stress-induced cell death. TRP voltage-independent calcium-permeable cation channels are divided into six subfamilies, two of which (TRPC and TRPM) have members that are widely expressed and activated by oxidative stress. Is activated by reactive oxygen/nitrogen species, resulting in cation conductance and anoxic neuronal cell death. This phenotype is rescued by suppression of ChaK1 expression. Related to ChaK2, another Atypical alpha kinase fused to a TRP ion channel protein. Protein type: Membrane protein, integral; Protein kinase, atypical; EC 2.7.11.1; Channel, cation; Membrane protein, multi-pass; Kinase, protein; ATYPICAL group; Alpha family; ChaK subfamily. Chromosomal Location of Human Ortholog: 15q21. Cellular Component: ruffle; integral to membrane; plasma membrane. Molecular Function: protein serine/threonine kinase activity; myosin binding; metal ion binding; calcium channel activity; actin binding; ATP binding. Biological Process: protein amino acid autophosphorylation; actomyosin structure organization and biogenesis; calcium-dependent cell-matrix adhesion; transmembrane transport. Disease: Amyotrophic Lateral Sclerosis-parkinsonism/dementia Complex 1

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage