CALM1 Antibody (C-term) | MyBioSource MBS9214524 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CALM1 Antibody (C-term)

catalog :

MBS9214524

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of CALM1 Antibody (C-term) in Hela cell line lysates (35ug/lane). CALM1 (arrow) was detected using the purified Pab.(2ug/ml)

image 2 :

CALM1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

image 3 :

CALM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9214524

products type :

Antibody

products full name :

CALM1 Antibody (C-term)

products short name :

[CALM1]

products name syn :

[Calmodulin; CaM; CALM1; CALM; CAM; CAM1]

other names :

[calmodulin isoform 2; Calmodulin; calmodulin; calmodulin 2 (phosphorylase kinase, delta)]

products gene name :

[CALM1]

other gene names :

[CALM2; CALM1; caM; PHKD; CAMII; LQT15; PHKD2; CALM; CAM; CAM1; CaM]

uniprot entry name :

CALM_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine, C Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Rat, Xenopus)

sequence positions :

[107-132]

sequence length :

149

specificity :

This CALM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-132 amino acids from the C-terminal region of human CALM1.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB~~1:1000. FC~~1:10~50. IHC-P~~1:50~100. IF~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Flow Cytometry (FC/FACS)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunofluorescence (IF)

image4 description :

Confocal immunofluorescent analysis of CALM1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

other info2 :

Cellular location: Cytoplasm, cytoskeleton, spindle. Cytoplasm,cytoskeleton, spindle pole. Note = Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. Function: Function Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

products categories :

Cancer; Cardiovascular; Cell Biology; Microbiology; Signal Transduction

products description :

CALM1 is a member of the EF-hand calcium-binding protein family.Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

products references :

Zhao,D., et.al., Zhonghua Yi Xue Za Zhi 88 (35), 2452-2456 (2008). Martins-de-Souza,D., et.al., J. Neural Transm. 116 (3), 275-289 (2009)

ncbi gi num :

4502549

ncbi acc num :

NP_001734.1

ncbi gb acc num :

NM_001743.5

uniprot acc num :

P62158

ncbi mol weight :

16838

ncbi pathways :

Arachidonic Acid Metabolism Pathway (805); Arachidonic Acid Metabolism Pathway (805); Arachidonic Acid Metabolism Pathway (805); Arachidonic Acid Metabolism Pathway (805); Arachidonic Acid Metabolism Pathway (805); Arachidonic Acid Metabolism Pathway (805); Arachidonic Acid Metabolism Pathway (805); Arachidonic Acid Metabolism Pathway (805); Arachidonic Acid Metabolism Pathway (805); Arachidonic Acid Metabolism Pathway (805)

ncbi summary :

This gene is a member of the calmodulin gene family. There are three distinct calmodulin genes dispersed throughout the genome that encode the identical protein, but differ at the nucleotide level. Calmodulin is a calcium binding protein that plays a role in signaling pathways, cell cycle progression and proliferation. Several infants with severe forms of long-QT syndrome (LQTS) who displayed life-threatening ventricular arrhythmias together with delayed neurodevelopment and epilepsy were found to have mutations in either this gene or another member of the calmodulin gene family (PMID:23388215). Mutations in this gene have also been identified in patients with less severe forms of LQTS (PMID:24917665), while mutations in another calmodulin gene family member have been associated with catecholaminergic polymorphic ventricular tachycardia (CPVT)(PMID:23040497), a rare disorder thought to be the cause of a significant fraction of sudden cardiac deaths in young individuals. Pseudogenes of this gene are found on chromosomes 10, 13, and 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

uniprot summary :

Calmodulin: a calcium-binding small regulatory protein that mediates the control of a large number of enzymes by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Has four functional calcium-binding domains. Phosphorylation and ubiquitination result in a decreased activity. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 14q32.11. Cellular Component: nucleoplasm; spindle pole; centrosome; sarcomere; growth cone; spindle microtubule; cytoplasm; plasma membrane; extracellular region; cytosol; nucleus; vesicle. Molecular Function: protein domain specific binding; nitric-oxide synthase regulator activity; titin binding; calcium ion binding; calcium-dependent protein binding; protein kinase binding; protein serine/threonine kinase activator activity; protein binding; N-terminal myristoylation domain binding; phospholipase binding; thioesterase binding; type 3 metabotropic glutamate receptor binding; phosphoinositide 3-kinase binding; nitric-oxide synthase binding; adenylate cyclase binding. Biological Process: positive regulation of phosphoprotein phosphatase activity; phototransduction, visible light; inositol phosphate metabolic process; nerve growth factor receptor signaling pathway; pathogenesis; signal transduction; nitric oxide metabolic process; positive regulation of protein amino acid dephosphorylation; synaptic transmission; platelet degranulation; substantia nigra development; muscle contraction; positive regulation of cyclic nucleotide metabolic process; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; platelet activation; fibroblast growth factor receptor signaling pathway; glycogen catabolic process; regulation of heart rate; positive regulation of nitric-oxide synthase activity; organelle organization and biogenesis; response to amphetamine; adenylate cyclase activation; glucose metabolic process; regulation of cytokinesis; positive regulation of protein amino acid autophosphorylation; rhodopsin mediated signaling; G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling; phospholipase C activation; carbohydrate metabolic process; innate immune response; positive regulation of cyclic-nucleotide phosphodiesterase activity; detection of calcium ion; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; response to calcium ion. Disease: Long Qt Syndrome 14; Ventricular Tachycardia, Catecholaminergic Polymorphic, 4

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage