BCOR Antibody (Center S1122) | MyBioSource MBS9214388 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

BCOR Antibody (Center S1122)

catalog :

MBS9214388

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of BCOR Antibody (Center S1122) in 293 cell line lysates (35ug/lane). BCOR (arrow) was detected using the purified Pab.

image 2 :

Western blot analysis of BCOR Antibody (Center S1122) in mouse stomach tissue lysates (35ug/lane). BCOR (arrow) was detected using the purified Pab.

image 3 :

BCOR Antibody (Center S1122) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCOR Antibody (Center S1122) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9214388

products type :

Antibody

products full name :

BCOR Antibody (Center S1122)

products short name :

[BCOR]

products name syn :

[BCL-6 corepressor; BCoR; BCOR; KIAA1575]

other names :

[BCL-6 corepressor isoform a; BCL-6 corepressor; BCL-6 corepressor; BCL6 corepressor]

products gene name :

[BCOR]

other gene names :

[BCOR; BCOR; MAA2; ANOP2; MCOPS2; KIAA1575; BCoR]

uniprot entry name :

BCOR_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[1107-1137]

sequence length :

1,703

specificity :

This BCOR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1107-1137 amino acids from the Central region of human BCOR.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

app notes :

WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

BCOR Antibody (Center S1122) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

products description :

BCOR was identified as an interacting corepressor of BCL6, a POZ/zinc finger transcription repressor that is required for germinal center formation and may influence apoptosis. This protein selectively interacts with the POZ domain of BCL6, but not with eight other POZ proteins. Specific class I and II histone deacetylases (HDACs) have been shown to interact with this protein, which suggests a possible link between the two classes of HDACs.

products references :

Ghetu,A.F., Mol. Cell 29 (3), 384-391 (2008). Hilton,E.N., Hum. Mol. Genet. 16 (14), 1773-1782 (2007)

ncbi gi num :

183396783

ncbi acc num :

NP_001116855.1

ncbi gb acc num :

NM_001123383.1

uniprot acc num :

Q6W2J9

ncbi mol weight :

192189

ncbi pathways :

Inositol Phosphate Metabolism Pathway (54880)

ncbi summary :

The protein encoded by this gene was identified as an interacting corepressor of BCL6, a POZ/zinc finger transcription repressor that is required for germinal center formation and may influence apoptosis. This protein selectively interacts with the POZ domain of BCL6, but not with eight other POZ proteins. Specific class I and II histone deacetylases (HDACs) have been shown to interact with this protein, which suggests a possible link between the two classes of HDACs. Several transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome Y.[provided by RefSeq, Jun 2010]

uniprot summary :

BCOR: Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem cells; the function implies inhibition of methylation on histone H3 Lys-4 (H3K4me3) and Lys-36 (H3K36me2). Defects in BCOR are the cause of microphthalmia syndromic type 2 (MCOPS2). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS2 is a very rare multiple congenital anomaly syndrome characterized by eye anomalies (congenital cataract, microphthalmia, or secondary glaucoma), facial abnormalities (long narrow face, high nasal bridge, pointed nose with cartilages separated at the tip, cleft palate, or submucous cleft palate), cardiac anomalies (atrial septal defect, ventricular septal defect, or floppy mitral valve) and dental abnormalities (canine radiculomegaly, delayed dentition, oligodontia, persistent primary teeth, or variable root length). Belongs to the BCOR family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: Xp11.4. Cellular Component: PcG protein complex; nucleus. Molecular Function: protein binding; histone deacetylase binding; heat shock protein binding; ubiquitin-protein ligase activity; transcription corepressor activity; transcription factor binding. Biological Process: odontogenesis; negative regulation of bone mineralization; transcription, DNA-dependent; negative regulation of histone H3-K36 methylation; heart development; specification of axis polarity; negative regulation of histone H3-K4 methylation; palate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent. Disease: Microphthalmia, Syndromic 2; Microphthalmia, Syndromic 1

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage