Phospho-AKT3 (S472) Antibody | MyBioSource MBS9213634 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-AKT3 (S472) Antibody

catalog :

MBS9213634

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

ELISA, enzyme immunoassay, dot blot

more info or order :

MyBioSource product webpage

image

image 1 :

Dot blot analysis of anti-AKT3-pS472 Phospho-specific Pab (RB13331) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

product information

catalog number :

MBS9213634

products type :

Antibody

products full name :

Phospho-AKT3 (S472) Antibody

products short name :

[Phospho-AKT3 (S472)]

products name syn :

[RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG]

other names :

[RAC-gamma serine/threonine-protein kinase isoform 2; RAC-gamma serine/threonine-protein kinase; RAC-gamma serine/threonine-protein kinase; v-akt murine thymoma viral oncogene homolog 3; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2]

products gene name :

[AKT3]

other gene names :

[AKT3; AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma; PKBG; PKB gamma]

uniprot entry name :

AKT3_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human . Predicted: Mouse, Rat.

sequence length :

465

specificity :

This AKT3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S472 of human AKT3.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles .

tested application :

Dot Blot (DB), ELISA (EIA)

app notes :

DB~~1:500

image1 heading :

Dot Blot (DB)

other info2 :

Function: AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis. Cellular location: Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tissue location: In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not atall in kidney.

products categories :

Cancer; Cell Biology; Immunology; Signal Transduction; Amyotrophic Lateral Sclerosis (ALS)

products description :

AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).

products references :

Xu, Z., et al., Biochem. Biophys. Res. Commun. 312(2):388-396 (2003). Tiwari, G., et al., Mol. Cancer Res. 1(6):475-484 (2003). Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003). Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002). Brodbeck, D., et al., J. Biol. Chem. 276(31):29550-29558 (2001).

ncbi gi num :

332078559

ncbi acc num :

NP_001193658.1

ncbi gb acc num :

NM_001206729.1

uniprot acc num :

Q9Y243

ncbi summary :

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described. Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; AGC group; AKT family. Chromosomal Location of Human Ortholog: 1q44. Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane. Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity. Biological Process: mitochondrial genome maintenance; signal transduction; protein amino acid phosphorylation. Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage