Mouse Heat shock protein HSP 90-beta, HSP90AB1 ELISA Kit | MyBioSource MBS921345 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Heat shock protein HSP 90-beta, HSP90AB1 ELISA Kit

catalog :

MBS921345

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS921345

products type :

ELISA Kit

products full name :

Mouse Heat shock protein HSP 90-beta, HSP90AB1 ELISA Kit

products short name :

heat shock protein 90kDa alpha (cytosolic) , class B member 1

products name syn :

Mouse Heat shock protein HSP 90-beta (HSP90AB1) ELISA kit; RP1-302G2.1; D6S182; FLJ26984; HSP90-BETA; HSP90B; HSPC2; HSPCB; OTTHUMP00000016518; OTTHUMP00000016519; heat shock 90kD protein 1; beta; heat shock 90kDa protein 1; beta; heat shock protein beta; heat shock protein 90kDa alpha (cytosolic); class B member 1

other names :

heat shock protein HSP 90-beta; Heat shock protein HSP 90-beta; heat shock protein HSP 90-beta; TSTA; HSP 84; heat shock 84 kDa; heat shock protein 1, beta; heat shock protein, 84 kDa 1; heat shock 90kDa protein 1, beta; retinal degeneration slow protein; tumor-specific transplantation 84 kDa antigen; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90 alpha (cytosolic), class B member 1; Heat shock 84 kDa; HSP 84; HSP84; Tumor-specific transplantation 84 kDa antigen; TSTA

products gene name :

HSP90AB1

other gene names :

Hsp90ab1; Hsp90ab1; 90kDa; Hsp84; Hsp90; Hspcb; C81438; Hsp84-1; AL022974; Hsp84; Hsp84-1; Hspcb; HSP 84; HSP84; TSTA

uniprot entry name :

HS90B_MOUSE

reactivity :

Mouse

sequence length :

724

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse HSP90AB1. No significant cross-reactivity or interference between mouse HSP90AB1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse HSP90AB1 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSP90AB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP90AB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP90AB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP90AB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

40556608

ncbi acc num :

NP_032328.2

ncbi gb acc num :

NM_008302.3

uniprot acc num :

P11499

ncbi mol weight :

83,281 Da

ncbi pathways :

Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Axon Guidance Pathway (575211); Developmental Biology Pathway (575210); NOD-like Receptor Signaling Pathway (122195); NOD-like Receptor Signaling Pathway (122131); Pathways In Cancer (83298); Progesterone-mediated Oocyte Maturation Pathway (119305); Progesterone-mediated Oocyte Maturation Pathway (503); Prostate Cancer Pathway (83304)

uniprot summary :

HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family. Protein type: Heat shock protein; Chaperone. Cellular Component: signalosome; cell surface; mitochondrion; membrane; brush border membrane; basolateral plasma membrane; apical plasma membrane; cytoplasm; intracellular; inclusion body; cytosol. Molecular Function: protein binding; GTP binding; double-stranded RNA binding; unfolded protein binding; dATP binding; nucleotide binding; sulfonylurea receptor binding; protein kinase binding; kinase binding; ATP binding. Biological Process: positive regulation of protein binding; positive regulation of protein import into nucleus, translocation; protein folding; positive regulation of cell size; response to stress; negative regulation of neuron apoptosis; placenta development

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage