ENPP2 Antibody (Center K416) | MyBioSource MBS9213445 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ENPP2 Antibody (Center K416)

catalog :

MBS9213445

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, cow

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of anti-ENPP2 Antibody (Center K416) in Y79 cell line lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.

image 2 :

Western blot analysis of anti-ENPP2 Antibody (Center K416) in mouse cerebellum tissue lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.

image 3 :

ENPP2 Antibody (Center K416) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENPP2 Antibody (Center K416) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9213445

products type :

Antibody

products full name :

ENPP2 Antibody (Center K416)

products short name :

[ENPP2]

products name syn :

[Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD; ENPP2; ATX; PDNP2]

other names :

[ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2 preproprotein; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ectonucleotide pyrophosphatase/phosphodiesterase 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD]

products gene name :

[ENPP2]

other gene names :

[ENPP2; ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA; ATX; PDNP2; E-NPP 2; LysoPLD]

uniprot entry name :

ENPP2_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine)

sequence positions :

[401-430]

sequence length :

888

specificity :

This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the Central region of human ENPP2.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P, FC, E

app notes :

WB~~1:1000. IHC-P~~1:10~50. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

ENPP2 Antibody (Center K416) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info2 :

Function: Hydrolyzes lysophospholipids to producelysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvementin cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor . Cellular location: Secreted. Note=Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells . Tissue Location: Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytesonly express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues

products categories :

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction

products description :

ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. This protein is secreted and further processed to make the biologically active form.

products references :

Kawagoe H., Soma O., Goji J., Nishimura N., Narita M.,Genomics 30:380-384(1995). Nam S.W., Clair T., Kim Y.S., McMarlin A.,Cancer Res. 61:6938-6944(2001). The MGC Project Team Genome Res. 14:2121-2127(2004)

ncbi gi num :

91823602

ncbi acc num :

NP_001035181.1

ncbi gb acc num :

NM_001040092.2

uniprot acc num :

Q13822

ncbi mol weight :

98994

ncbi summary :

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]

uniprot summary :

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.4.39; Secreted; Secreted, signal peptide; Lipid Metabolism - ether lipid; Motility/polarity/chemotaxis; Phosphodiesterase. Chromosomal Location of Human Ortholog: 8q24.1. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane. Molecular Function: phosphodiesterase I activity; nucleotide diphosphatase activity; nucleic acid binding; hydrolase activity; zinc ion binding; calcium ion binding; transcription factor binding; alkylglycerophosphoethanolamine phosphodiesterase activity; scavenger receptor activity; polysaccharide binding; lysophospholipase activity. Biological Process: G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; phospholipid catabolic process; immune response; chemotaxis; cell motility; phosphate metabolic process; regulation of cell migration; regulation of angiogenesis

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage