catalog number :
MBS921337
products type :
ELISA Kit
products full name :
Human Thromboxane A2 receptor, TBXA2R ELISA Kit
products short name :
thromboxane A2 receptor
products name syn :
Human Thromboxane A2 receptor (TBXA2R) ELISA kit; TXA2-R; prostanoid TP receptor; thromboxane A2 receptor
other names :
thromboxane A2 receptor; Thromboxane A2 receptor; thromboxane A2 receptor; TXA2-R; prostanoid TP receptor; thromboxane A2 receptor; Prostanoid TP receptor
products gene name :
TBXA2R
other gene names :
TBXA2R; TBXA2R; TXA2-R
uniprot entry name :
TA2R_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human TBXA2R. No significant cross-reactivity or interference between Human TBXA2R and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 25 pg/ml-1600 pg/ml. Sensitivity: 6.25 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TBXA2R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TBXA2R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TBXA2R is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TBXA2R bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001161391.1
ncbi gb acc num :
NM_001167919.1
ncbi mol weight :
37,841 Da
ncbi pathways :
Calcium Signaling Pathway (84289); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (581436); Eicosanoid Ligand-binding Receptors Pathway (581449); G Alpha (12/13) Signalling Events Pathway (581468); G Alpha (q) Signalling Events Pathway (581465); GPCR Downstream Signaling Pathway (581461); GPCR Ligand Binding Pathway (581435); Hemostasis Pathway (581607); Neuroactive Ligand-receptor Interaction Pathway (84292)
uniprot summary :
Function: Receptor for thromboxane A2 (TXA2), a potent stimulator of platelet aggregation. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. In the kidney, the binding of TXA2 to glomerular TP receptors causes intense vasoconstriction. Activates phospholipase C and adenylyl cyclase.
size4 :
10x96-Strip-Wells