ELISA/assay

Human Acyloxyacyl hydrolase, AOAH ELISA Kit

MBS921316

48-Strip-Wells

510 USD

ELISA Kit

Human Acyloxyacyl hydrolase, AOAH ELISA Kit

acyloxyacyl hydrolase (neutrophil)

Human Acyloxyacyl hydrolase (AOAH) ELISA kit; acyloxyacyl hydrolase; acyloxyacyl hydrolase (neutrophil)

acyloxyacyl hydrolase isoform 2 preproprotein; Acyloxyacyl hydrolase; acyloxyacyl hydrolase; acyloxyacyl hydrolase (neutrophil)

AOAH

AOAH; AOAH

AOAH_HUMAN

Human

688

This assay has high sensitivity and excellent specificity for detection of human AOAH. No significant cross-reactivity or interference between human AOAH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AOAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AOAH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AOAH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AOAH bound in the initial step. The color development is stopped and the intensity of the color is measured.

294610665

NP_001170977.1

NM_001177506.1

P28039

65,105 Da

This locus encodes both the light and heavy subunits of acyloxyacyl hydrolase. The encoded enzyme catalyzes the hydrolysis of acyloxylacyl-linked fatty acyl chains from bacterial lipopolysaccharides, effectively detoxifying these molecules. The encoded protein may play a role in modulating host inflammatory response to gram-negative bacteria. Alternatively spliced transcript variants have been described.[provided by RefSeq, Apr 2010]

AOAH: Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.1.77; Secreted, signal peptide; Lipase; Secreted. Chromosomal Location of Human Ortholog: 7p14.2. Cellular Component: extracellular region. Molecular Function: lipoprotein lipase activity; acyloxyacyl hydrolase activity; catalytic activity. Biological Process: lipid metabolic process; inflammatory response

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800