antibody

CHUK Antibody (N-term)

MBS9212858

0.08 mL

165 USD

polyclonal

rabbit

nonconjugated

human, mouse, chicken

western blot, ELISA, enzyme immunoassay

Antibody

CHUK Antibody (N-term)

[CHUK]

[Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16; CHUK; IKKA; TCF16]

[inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit alpha; inhibitor of nuclear factor kappa-B kinase subunit alpha; conserved helix-loop-helix ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16]

[CHUK]

[CHUK; CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha; IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16]

IKKA_HUMAN

Polyclonal

Rabbit Ig

Rabbit

Human (Predicted Reactivity: Bovine, Chicken, Mouse)

[84-112]

745

This CHUK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the N-terminal region of human CHUK.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB), ELISA (EIA)

WB~~1:1000

Western Blot (WB)

Function: Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa- B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non -canonicalNF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Cellular Location: Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus. Tissue Location: Widely expressed.

Cardiovascular; Cell Biology; Immunology; Signal Transduction

This gene encodes a member of the serine/threonine protein. kinase family. The encoded protein, a component of a. cytokine-activated protein complex that is an inhibitor of the. essential transcription factor NF-kappa-B complex, phosphorylates. sites that trigger the degradation of the inhibitor via the. ubiquination pathway, thereby activating the transcription factor.

Lahtela, J., et al. N. Engl. J. Med. 363(17):1631-1637(2010). Gouin, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(40):17333-17338(2010). Cummins, E.P., et al. J. Immunol. 185(7):4439-4445(2010). Li, T., et al. Nat. Immunol. 11(9):799-805(2010). Rotman, Y., et al. Hepatology 52(3):894-903(2010)

62241001

NP_001269.3

NM_001278.3

O15111

84640

Glycine, Serine And Threonine Metabolism Pathway (1147); Glycine, Serine And Threonine Metabolism Pathway (1147); Glycine, Serine And Threonine Metabolism Pathway (1147); Glycine, Serine And Threonine Metabolism Pathway (1147); Glycine, Serine And Threonine Metabolism Pathway (1147); Glycine, Serine And Threonine Metabolism Pathway (1147); Glycine, Serine And Threonine Metabolism Pathway (1147); Glycine, Serine And Threonine Metabolism Pathway (1147); Glycine, Serine And Threonine Metabolism Pathway (1147); Glycine, Serine And Threonine Metabolism Pathway (1147)

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541. Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.10; Kinase, protein; Protein kinase, Other; Other group; IKK family. Chromosomal Location of Human Ortholog: 10q24-q25. Cellular Component: nucleoplasm; internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol. Molecular Function: protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; ATP binding; protein kinase activity. Biological Process: lactation; skeletal muscle contraction; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; response to toxin; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; osteoclast differentiation; protein amino acid phosphorylation; T cell receptor signaling pathway; Rho protein signal transduction; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; morphogenesis of an epithelial sheet; inflammatory response; toll-like receptor 4 signaling pathway; response to drug; epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; response to virus; MyD88-independent toll-like receptor signaling pathway; mammary gland epithelial cell proliferation; response to amino acid stimulus; odontogenesis of dentine-containing teeth; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to hydroperoxide; toll-like receptor signaling pathway; innate immune response; striated muscle cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation. Disease: Cocoon Syndrome

0.08 mL

165 USD

0.4 mL

370