KLF9 Antibody (N-term) | MyBioSource MBS9212765 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

KLF9 Antibody (N-term)

catalog :

MBS9212765

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-KLF9 Antibody (N-term) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Anti-KLF9 Antibody (N-term) at 1:2000 dilution+ U-87 MG whole cell lysate Lysates/proteins at20 µg per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

KLF9 Antibody (N-term)western blot analysis in WiDr cell line lysates(35ug/lane).This demonstrates the KLF9antibody detected the KLF9 protein (arrow).

product information

catalog number :

MBS9212765

products type :

Antibody

products full name :

KLF9 Antibody (N-term)

products short name :

[KLF9]

products name syn :

[Krueppel-like factor 9; Basic transcription element-binding protein 1; BTE-binding protein 1; GC-box-binding protein 1; Transcription factor BTEB1; KLF9; BTEB; BTEB1]

other names :

[Krueppel-like factor 9; Krueppel-like factor 9; Krueppel-like factor 9; Kruppel-like factor 9; Basic transcription element-binding protein 1; BTE-binding protein 1; GC-box-binding protein 1; Transcription factor BTEB1]

products gene name :

[KLF9]

other gene names :

[KLF9; KLF9; BTEB; BTEB1; BTEB; BTEB1; BTE-binding protein 1]

uniprot entry name :

KLF9_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Mouse, Pig, Rat)

sequence positions :

[29-57]

specificity :

This KLF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human KLF9.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)

app notes :

WB~~1:1000. IF~~1;10~50

image1 heading :

Testing Data

image2 heading :

Testing Data (TD)

image3 heading :

Testing Data (TD)

image4 heading :

Testing Data (TD)

image4 description :

Fluorescent confocal image of U251 cellstained with KLF9 Antibody(N-term).U251 cells were fixedwith 4% PFA (20 min), permeabilized withTriton X-100 (0.1%, 10 min), then incubatedwith KLF9 primary antibody (1:25, 1 h at 37?).For secondary antibody, Alexa Fluor® 488conjugated donkey anti-rabbit antibody (green)was used (1:400, 50 min at 37?).Cytoplasmicactin was counterstained with Alexa Fluor®555 (red) conjugated Phalloidin (7units/ml, 1 hat 37?). Nuclei were counterstained with DAPI(blue) (10 µg/ml, 10 min). KLF9immunoreactivity is localized to vesicles andNucleus significantly.

other info1 :

Cellular location: nucleus. Tissue location: Epidermis (at protein level)

products description :

The protein encoded by this gene is a transcription factor. that binds to GC box elements located in the promoter. Binding of. the encoded protein to a single GC box inhibits mRNA expression. while binding to tandemly repeated GC box elements activates. transcription.

products references :

Du, H., et al. Biol. Reprod. 83(2):205-211(2010). Pabona, J.M., et al. Endocrinology 151(7):3396-3406(2010). Gamper, I., et al. Exp. Hematol. 37(5):539-548(2009). Kang, L., et al. Pathol. Int. 58(6):334-338(2008). Simmen, F.A., et al. Reprod. Biol. Endocrinol. 6, 41 (2008) :

ncbi gi num :

4557375

ncbi acc num :

NP_001197.1

ncbi gb acc num :

NM_001206.2

uniprot acc num :

Q13886

ncbi pathways :

Oxidative Phosphorylation Pathway (687)

ncbi summary :

The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription. [provided by RefSeq, Jul 2008]

uniprot summary :

KLF9: Transcription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box. Belongs to the Sp1 C2H2-type zinc-finger protein family. Protein type: C2H2-type zinc finger protein; DNA-binding. Chromosomal Location of Human Ortholog: 9q13. Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus. Molecular Function: DNA binding; metal ion binding; transcription factor activity. Biological Process: circadian rhythm; regulation of transcription from RNA polymerase II promoter; progesterone receptor signaling pathway; transcription, DNA-dependent; embryo implantation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage