PI3KC3 Antibody (C-term E785) | MyBioSource MBS9212273 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PI3KC3 Antibody (C-term E785)

catalog :

MBS9212273

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysate from U-251 MG cell line, using hPI3KC3 (C-term). MBS9212273 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

image 2 :

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with hPI3KC3 (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

product information

catalog number :

MBS9212273

products type :

Antibody

products full name :

PI3KC3 Antibody (C-term E785)

products short name :

[PI3KC3]

products name syn :

[Phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3; VPS34]

other names :

[phosphatidylinositol 3-kinase catalytic subunit type 3 isoform 1; Phosphatidylinositol 3-kinase catalytic subunit type 3; phosphatidylinositol 3-kinase catalytic subunit type 3; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34]

products gene name :

[PIK3C3]

other gene names :

[PIK3C3; PIK3C3; VPS34; Vps34; hVps34; VPS34; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3]

uniprot entry name :

PK3C3_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Mouse, Pig, Rat)

sequence positions :

[770-801]

sequence length :

887

specificity :

This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-801 amino acids from the C-terminal region of human PI3KC3.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

ELISA (EIA), Immunohistochemistry-Paraffin (IHC), Western Blot (WB)

app notes :

WB~~1:1000. IHC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Function: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol3-phosphate; different complex forms arebelieved to play a role in multiple membranetrafficking pathways: PI3KC3-C1 is involved ininitiation of autophagosomes and PI3KC3-C2in maturation of autophagosomes andendocytosis. Involved in regulation ofdegradative endocytic trafficking andrequired for the abcission step in cytokinesis,probably in the context of PI3KC3-C2. Cellular location: Midbody. Late endosome. Cytoplasmicvesicle, autophagosome. Note=As componentof the PI3K complex I localized topre-autophagosome structures. Ascomponent of the PI3K complex II localizedpredominantly to endosomes (Probable).Localizes also to discrete punctae along theciliary axoneme and to the base of the ciliaryaxoneme . Tissue location: Ubiquitously expressed, with a highestexpression in skeletal muscle.

products categories :

Immunology; Metabolism; Signal Transduction; Autophagy

products description :

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

products references :

Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003). Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).

ncbi gi num :

34761064

ncbi acc num :

NP_002638.2

ncbi gb acc num :

NM_002647.3

uniprot acc num :

Q8NEB9

ncbi pathways :

Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289)

uniprot summary :

PIK3C3: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Ubiquitously expressed, with a highest expression in skeletal muscle. Belongs to the PI3/PI4-kinase family. Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; Autophagy; EC 2.7.1.137; Kinase, lipid. Chromosomal Location of Human Ortholog: 18q12.3. Cellular Component: membrane; late endosome; midbody; phagocytic vesicle; axoneme; phosphoinositide 3-kinase complex; cytosol. Molecular Function: protein binding; 1-phosphatidylinositol-3-kinase activity; ATP binding; protein kinase activity. Biological Process: phosphoinositide-mediated signaling; endosome organization and biogenesis; cytokinesis; protein amino acid phosphorylation; response to leucine; regulation of protein secretion; early endosome to late endosome transport; phosphoinositide phosphorylation; phospholipid metabolic process; insulin receptor signaling pathway; toll-like receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; protein processing; toll-like receptor 9 signaling pathway; autophagic vacuole formation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage