GLD2 Antibody (N-term) | MyBioSource MBS9212203 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GLD2 Antibody (N-term)

catalog :

MBS9212203

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded H. cerebellum section using GLD2 Antibody (N-term). MBS9212203 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

image 2 :

Fluorescent image of MCF-7 cells stained with GLD2 Antibody (N-term). MBS9212203 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

image 3 :

Western blot analysis of lysates from mouse brain, mouse cerebellum tissue lysate (from left to right), using GLD2 Antibody (N-term). MBS9212203 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

product information

catalog number :

MBS9212203

products type :

Antibody

products full name :

GLD2 Antibody (N-term)

products short name :

[GLD2]

products name syn :

[Poly(A) RNA polymerase GLD2; hGLD-2; PAP-associated domain-containing protein 4; Terminal uridylyltransferase 2; TUTase 2; PAPD4; GLD2]

other names :

[poly(A) RNA polymerase GLD2 isoform 1; Poly(A) RNA polymerase GLD2; poly(A) RNA polymerase GLD2; PAP associated domain containing 4; PAP-associated domain-containing protein 4; Terminal uridylyltransferase 2; TUTase 2]

products gene name :

[PAPD4]

other gene names :

[PAPD4; PAPD4; GLD2; TUT2; GLD2; hGLD-2; TUTase 2]

uniprot entry name :

GLD2_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, Mouse (Predicted Reactivity: Bovine)

sequence positions :

[58-87]

sequence length :

480

specificity :

This GLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human GLD2.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

ELISA (EIA), Immunohistochemistry (IHC-P), Immunofluorescence (IF), Western Blot (WB)

app notes :

IF~~1:25. IHC-P~~1:50~100. WB~~1:1000

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of lysates from HepG2 cell line and mouse brain tissue lysate (from left to right), using GLD2 Antibody (N-term). MBS9212203 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

image5 heading :

Immunohistochemistry (IHC)

image5 description :

GLD2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

other info1 :

Cellular Location: Cytoplasm. Nucleus. Tissue Location: Expressed in brain. Within brain, it is expressed in cerebellum, hippocampus and medulla. Antigen Region: 58-87

products categories :

Crown Antibodies

products description :

GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.

products references :

Mullen, T.E., et al. Genes Dev. 22(1):50-65(2008) Kwak, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 101(13):4407-4412(2004)

ncbi gi num :

167555097

ncbi acc num :

NP_001107865.1

ncbi gb acc num :

NM_001114393.1

uniprot acc num :

Q6PIY7

ncbi mol weight :

56028

uniprot summary :

PAPD4: Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3 -end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation. Belongs to the DNA polymerase type-B-like family. GLD2 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.7.19; RNA processing; Transferase. Chromosomal Location of Human Ortholog: 5q14.1. Cellular Component: cytoplasm. Molecular Function: polynucleotide adenylyltransferase activity; metal ion binding; ATP binding. Biological Process: RNA polyadenylation; hemopoietic progenitor cell differentiation; mRNA processing

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage