RNF43 Antibody (C-term) | MyBioSource MBS9212124 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RNF43 Antibody (C-term)

catalog :

MBS9212124

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-RNF43 Antibody (C-term) at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

RNF43 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RNF43 antibody detected the RNF43 protein (arrow).

image 3 :

RNF43 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9212124

products type :

Antibody

products full name :

RNF43 Antibody (C-term)

products short name :

[RNF43]

products name syn :

[E3 ubiquitin-protein ligase RNF43; 632-; RING finger protein 43; RNF43]

other names :

[E3 ubiquitin-protein ligase RNF43 isoform 1; E3 ubiquitin-protein ligase RNF43; E3 ubiquitin-protein ligase RNF43; ring finger protein 43; RING finger protein 43]

products gene name :

[RNF43]

other gene names :

[RNF43; RNF43; URCC; RNF124]

uniprot entry name :

RNF43_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[543-572]

sequence length :

869

specificity :

This RNF43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human RNF43.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

app notes :

WB~~1:1000. IHC-P~~1:10~50

image1 heading :

Western Blot

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN. Function: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, there by resticting the size of the intestinal stem cell zone. Cellular Location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope. Note=According to a report, maybe secreted. Tissue Location: Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines.

products categories :

Cancer; Cell Biology

products description :

RNF43 is a HAP95 (AKAP8L; MIM 609475) binding ubiquitin. ligase that promotes cell growth and is upregulated in colon cancer. (Yagyu et al., 2004 [PubMed 15492824]; Sugiura et al., 2008 [PubMed. 18313049]).

products references :

Low, S.K., et al. PLoS ONE 5 (7), E11824 (2010) :. Sugiura, T., et al. Exp. Cell Res. 314(7):1519-1528(2008). Uchida, N., et al. Clin. Cancer Res. 10(24):8577-8586(2004). Yagyu, R., et al. Int. J. Oncol. 25(5):1343-1348(2004)

ncbi gi num :

56711322

ncbi acc num :

NP_060233.3

ncbi gb acc num :

NM_017763.5

uniprot acc num :

Q68DV7

ncbi mol weight :

85722

ncbi pathways :

Methane Metabolism Pathway (54894); Methane Metabolism Pathway (54894); Methane Metabolism Pathway (54894); Methane Metabolism Pathway (54894); Methane Metabolism Pathway (54894); Methane Metabolism Pathway (54894); Methane Metabolism Pathway (54894); Methane Metabolism Pathway (54894)

ncbi summary :

The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

uniprot summary :

RNF43: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. Belongs to the ZNRF3 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin ligase; Membrane protein, integral; Ubiquitin conjugating system; EC 6.3.2.-; Ligase. Chromosomal Location of Human Ortholog: 17q22. Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; nuclear envelope. Molecular Function: protein binding; zinc ion binding; frizzled binding; ubiquitin-protein ligase activity; ligase activity. Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage