ENOB Antibody (Center) | MyBioSource MBS9212064 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ENOB Antibody (Center)

catalog :

MBS9212064

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, flow cytometry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

ENOB Antibody (Center) western blot analysis in RD cell line and mouse skeletal muscle,rat skeletal muscle lysates (35ug/lane).This demonstrates the ENOB antibody detected the ENOB protein (arrow).

image 2 :

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 3 :

ENOB Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9212064

products type :

Antibody

products full name :

ENOB Antibody (Center)

products short name :

[ENOB]

products name syn :

[Beta-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; MSE; Skeletal muscle enolase; ENO3]

other names :

[beta-enolase isoform 2; Beta-enolase; beta-enolase; enolase 3 (beta, muscle); 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; MSE; Skeletal muscle enolase]

products gene name :

[ENO3]

other gene names :

[ENO3; ENO3; MSE; GSD13; MSE]

uniprot entry name :

ENOB_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)

sequence positions :

[237-264]

sequence length :

391

specificity :

This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry- Paraffin, Flow Cytometry (FC), ELISA (EIA)

app notes :

WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Calculated MW: 46987

other info2 :

Function: Appears to have a function in striated muscle development and regeneration. Cellular Location: Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity. Tissue Location: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

products categories :

Crown Antibodies; Cancer; Metabolism; Signal Transduction

products description :

ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.

products references :

Aurino,S., Acta Myol 27, 90-97 (2008). Giallongo,A., Eur. J. Biochem. 214 (2), 367-374 (1993)

ncbi gi num :

301897479

ncbi acc num :

NP_001180432.1

ncbi gb acc num :

NM_001193503.1

uniprot acc num :

P13929

ncbi pathways :

Fatty Acid Biosynthesis Pathway (2027); Fatty Acid Biosynthesis Pathway (2027); Fatty Acid Biosynthesis Pathway (2027); Fatty Acid Biosynthesis Pathway (2027); Fatty Acid Biosynthesis Pathway (2027); Fatty Acid Biosynthesis Pathway (2027); Fatty Acid Biosynthesis Pathway (2027); Fatty Acid Biosynthesis Pathway (2027); Fatty Acid Biosynthesis Pathway (2027); Fatty Acid Biosynthesis Pathway (2027)

ncbi summary :

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]

uniprot summary :

ENO3: Appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen- beta particles are detected on ultrastructural analysis. Belongs to the enolase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 4.2.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase. Chromosomal Location of Human Ortholog: 17p13.2. Cellular Component: extracellular space; phosphopyruvate hydratase complex; cytoplasm; plasma membrane; cytosol. Molecular Function: protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity. Biological Process: response to drug; glycolysis; carbohydrate metabolic process; skeletal muscle regeneration; glucose metabolic process; pathogenesis; aging; gluconeogenesis. Disease: Glycogen Storage Disease Xiii

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage