MAPE Antibody (C-term) | MyBioSource MBS9211992 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MAPE Antibody (C-term)

catalog :

MBS9211992

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Overlay histogram showing Hela cells stained with AP6720b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody MBS9211992, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image 2 :

All lanes : Anti-MAPE Antibody (C-term) at 1:2000 dilution . Lane 1: Hela whole cell lysate . Lane 2: K562 whole cell lysate. Lane 3: 293 whole cell lysate. Lane 4: human testis lysate . Lane 5: human brain lysate . Lane 6: human kidney lysate Lysates/proteins at 20 ug perlane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa . Blocking/Dilutionbuffer: 5% NFDM/TBST.

image 3 :

MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated

product information

catalog number :

MBS9211992

products type :

Antibody

products full name :

MAPE Antibody (C-term)

products short name :

[MAPE]

products name syn :

[Melanoma antigen preferentially expressed in tumors; Opa-interacting protein 4; OIP-4; Preferentially expressed antigen of melanoma; PRAME; MAPE; OIP4]

other names :

[melanoma antigen preferentially expressed in tumors isoform a; Melanoma antigen preferentially expressed in tumors; melanoma antigen preferentially expressed in tumors; preferentially expressed antigen in melanoma; Opa-interacting protein 4; OIP-4; Preferentially expressed antigen of melanoma]

products gene name :

[PRAME]

other gene names :

[PRAME; PRAME; MAPE; OIP4; CT130; OIP-4; MAPE; OIP4; OIP-4]

uniprot entry name :

PRAME_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[476-502]

sequence length :

509

specificity :

This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Flow Cytometry ( FC ), Western Blot ( WB ), Immunohistochemistry-P ( IHC-P ) , ELISA ( EIA )

app notes :

FC~~ 1:25. WB~~1:2000. IHC-P~~ 1:50~100

image1 heading :

Testing Data (TD)

image2 heading :

Testing Data (TD)

image3 heading :

Testing Data (TD)

other info2 :

Function: Functions as a transcriptional repressor,inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Cellular Location: Nucleus. Cell membrane . Tissue Location: Expressed in testis. Detected in samples of kidney, brain and skin

products categories :

Cancer

products description :

MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.

products references :

Rezvani,K., Blood 113 (10), 2245-2255 (2009). Qin,Y., Leuk. Res. 33 (3), 384-390 (2009). Neumann,E., Cancer Res. 58 (18), 4090-4095 (1998)

ncbi gi num :

5174641

ncbi acc num :

NP_006106.1

ncbi gb acc num :

NM_006115.4

uniprot acc num :

P78395

ncbi summary :

This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

uniprot summary :

PRAME: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Belongs to the PRAME family. Protein type: Transcription, coactivator/corepressor; Cancer Testis Antigen (CTA); Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 22q11.22. Cellular Component: plasma membrane; nucleus. Molecular Function: protein binding; retinoic acid receptor binding. Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell differentiation; transcription, DNA-dependent; apoptosis; regulation of growth; positive regulation of cell proliferation; cell differentiation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage