BDNF Antibody (C-term) | MyBioSource MBS9211799 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

BDNF Antibody (C-term)

catalog :

MBS9211799

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-BDNF Antibody (C-term) at 1:1000 dilution Lane 1: human cerebellum lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST

image 2 :

Formalin-fixed and paraffin-embedded human brain reacted with BDNF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 3 :

Confocal immunofluorescent analysis of BDNF Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

product information

catalog number :

MBS9211799

products type :

Antibody

products full name :

BDNF Antibody (C-term)

products short name :

[BDNF]

products name syn :

[Brain-derived neurotrophic factor; BDNF; Abrineurin; BDNF]

other names :

[brain-derived neurotrophic factor isoform a preproprotein; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; brain-derived neurotrophic factor; Abrineurin]

products gene name :

[BDNF]

other gene names :

[BDNF; BDNF; ANON2; BULN2; BDNF]

uniprot entry name :

BDNF_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Horse, Mouse, Pig, Rat

sequence positions :

[206-236]

sequence length :

247

specificity :

This BDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human BDNF.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P, IF, FC, E

app notes :

WB~~1:1000. IHC-P~~1:10~50. IF~~1:10~50. FC~~1:10~50

image1 heading :

Testing Data (TD)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

Flow cytometric analysis of CEM cells using BDNF Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Cellular location: Secreted. Tissue location: Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expresed in heart, lung , skeletal muscle, testis, prostate and placenta.

products categories :

Cardiovascular; Metabolism; Neuroscience; Signal Transduction

products description :

BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this protein is reduced in both Alzheimer's and Huntington disease patients. This protein may play a role in the regulation of stress response and in the biology of mood disorders.

products references :

Jiang,H., Clin. Chim. Acta 400 (1-2), 3-7 (2009). Liu,L., Psychiatr. Genet. 18 (6), 267-274 (2008). Dmitrzak-Weglarz,M., Pharmacogenomics 9 (11), 1595-1603 (2008)

ncbi gi num :

219842288

ncbi acc num :

NP_001137277.1

ncbi gb acc num :

NM_001143805.1

uniprot acc num :

P23560

ncbi pathways :

, Pathway (627); , Pathway (627); , Pathway (627); , Pathway (627); , Pathway (627); , Pathway (627); , Pathway (627); , Pathway (627); , Pathway (627); , Pathway (627)

ncbi summary :

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]

uniprot summary :

BDNF: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Belongs to the NGF-beta family. 5 isoforms of the human protein are produced by alternative promoter. Protein type: Cytokine; Secreted; Cell development/differentiation; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: extracellular space; synaptic vesicle; mitochondrial crista; perinuclear region of cytoplasm; cytoplasm; dendrite; cytoplasmic membrane-bound vesicle; extracellular region; terminal button; perikaryon. Molecular Function: growth factor activity; neurotrophin TRKB receptor binding. Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; behavioral fear response; mitochondrial electron transport, NADH to ubiquinone; regulation of neuron differentiation; axon extension; response to hormone stimulus; positive regulation of long-term neuronal synaptic plasticity; response to vitamin A; negative regulation of neuroblast proliferation; ureteric bud development; dendrite development; regulation of retinal cell programmed cell death; feeding behavior; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; response to electrical stimulus; inner ear development; response to food; nervous system development; chronic inflammatory response; response to light intensity; neuron recognition; regulation of axon extension; learning; regulation of short-term neuronal synaptic plasticity; negative regulation of striated muscle development; positive regulation of peptidyl-serine phosphorylation; positive regulation of synaptogenesis; axon target recognition; response to hyperoxia; glutamate secretion; response to fluoxetine; response to hypoxia; nerve development; response to activity; regulation of excitatory postsynaptic membrane potential; positive regulation of neuron differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis; gamma-aminobutyric acid signaling pathway. Disease: Obsessive-compulsive Disorder; Bulimia Nervosa, Susceptibility To, 1; Bulimia Nervosa, Susceptibility To, 2; Central Hypoventilation Syndrome, Congenital

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage