ALDH1A1 Antibody | MyBioSource MBS9211778 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ALDH1A1 Antibody

catalog :

MBS9211778

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[RB23017]

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysates from HepG2 cell line and human lung tissue lysate (from left to right), using ALDH1A1 Antibody. MBS9211778 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

image 2 :

Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9211778 ALDH1A1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.

image 3 :

ALDH1A1 Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9211778

products type :

Antibody

products full name :

ALDH1A1 Antibody

products short name :

[ALDH1A1]

products name syn :

[Retinal dehydrogenase 1; RALDH 1; RalDH1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase; cytosolic; ALDH1A1; ALDC; ALDH1; PUMB1]

other names :

[retinal dehydrogenase 1; Retinal dehydrogenase 1; retinal dehydrogenase 1; aldehyde dehydrogenase 1 family, member A1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic]

products gene name :

[ALDH1A1]

other gene names :

[ALDH1A1; ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e; ALDC; ALDH1; PUMB1; RALDH 1; RalDH1]

uniprot entry name :

AL1A1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

clone :

[RB23017]

host :

Rabbit

reactivity :

Human

sequence length :

501

specificity :

This ALDH1A1 antibody is generated from rabbits immunized with human ALDH1A1 recombinant protein.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC) - Paraffin, Flow Cytometry (FC/FACS), Western Blot (WB)

app notes :

WB~~1:1000. IF~~1:100. IHC-P~~1:50~100. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

ALDH1A1 Antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Function: Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (PubMed: 19296407 , PubMed:<a href="http://www.uniprot.org/citations/26373694" target="_blank">26373694 , PubMed: 25450233 ). Cellular Location: Cytoplasm, cytosol {ECO:0000250 UniProtKB:P48644}

products categories :

Crown Antibodies; Cancer; Signal Transduction

products description :

ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.

products references :

References for protein:. 1.Moore,S., J Stud Alcohol Drugs 68 (2), 192-196 (2007). 2.Collard,F., Biochimie 89 (3), 369-373 (2007). References for HepG2 cell line:. 1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960]. 2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851]. 3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761-1775. [PubMed:7506266]. 4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1-3): 329-340. [PubMed:15138059].

ncbi gi num :

21361176

ncbi acc num :

NP_000680.2

ncbi gb acc num :

NM_000689.4

uniprot acc num :

P00352

ncbi pathways :

Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216); Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216); Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216); Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216); Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216); Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216); Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216); Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216); Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216); Superpathway Of Pyrimidine Deoxyribonucleosides Degradation (216)

ncbi summary :

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]

uniprot summary :

ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family. Protein type: GAPs; GAPs, Ras; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; EC 1.2.1.36; Apoptosis. Chromosomal Location of Human Ortholog: 9q21.13. Cellular Component: cytoplasm; cytosol. Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity. Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage