HSF1 Sumoylation Site Antibody | MyBioSource MBS9211638 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HSF1 Sumoylation Site Antibody

catalog :

MBS9211638

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

HSF1 Antibody western blot analysis in Jurkat cell line and mouse heart tissue lysates (35ug/lane).This demonstrates the HSF1 antibody detected the HSF1 protein (arrow).

image 2 :

Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

product information

catalog number :

MBS9211638

products type :

Antibody

products full name :

HSF1 Sumoylation Site Antibody

products short name :

[HSF1 Sumoylation Site]

products name syn :

[Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1; HSF1; HSTF1]

other names :

[heat shock factor protein 1; Heat shock factor protein 1; heat shock factor protein 1; heat shock transcription factor 1; Heat shock transcription factor 1; HSTF 1]

products gene name :

[HSF1]

other gene names :

[HSF1; HSF1; HSTF1; HSTF1; HSF 1; HSTF 1]

uniprot entry name :

HSF1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse (Predicted Reactivity: Bovine)

sequence positions :

[278-309]

sequence length :

529

specificity :

This HSF1 Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-309 amino acids from human HSF1 Sumoylation Site.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunofluorescence (IF), ELISA (EIA), Western Blot (WB)

app notes :

WB~~1:1000. IF~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Function: Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage- dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner.

other info2 :

Cellular Location: Nucleus. Cytoplasm. Nucleus, nucleoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Note=The monomeric form is cytoplasmic in unstressed cells. Predominantly nuclear protein in both unstressed and heat shocked cells. Translocates in the nucleus upon heat shock. Nucleocytoplasmic shuttling protein. Colocalizes with IER5 in the nucleus. Colocalizes with BAG3 to the nucleus upon heat stress. Localizes in subnuclear granules called nuclear stress bodies (nSBs) upon heat shock. Colocalizes with SYMPK and SUMO1 in nSBs upon heat shock. Colocalizes with PRKACA/PKA in the nucleus and nSBs upon heat shock. Relocalizes from the nucleus to the cytoplasm during the attenuation and recovery phase period of the heat shock response. Translocates in the cytoplasm in a YWHAE- and XPO1/CRM1-dependent manner. Together with histone H2AX, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR). Colocalizes with calcium-responsive transactivator SS18L1 at kinetochore region on the mitotic chromosomes. Colocalizes with gamma tubulin at centrosome. Localizes at spindle pole in metaphase. Colocalizes with PLK1 at spindle poles during prometaphase.

products categories :

Crown Antibodies; Cardiovascular; Cell Biology; Neuroscience

products description :

Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.

products references :

Hilgarth, et al., Biochem Biophys Res Commun. 2003 Mar 28;303(1):196-200. He, H., et al., J. Biol. Chem. 278(37):35465-35475 (2003). Wang, X., et al., Mol. Cell. Biol. 23(17):6013-6026 (2003). Ignatenko, N.A., et al., Exp. Cell Res. 288(1):1-8 (2003). Soncin, F., et al., Biochem. Biophys. Res. Commun. 303(2):700-706 (2003).

ncbi gi num :

5031767

ncbi acc num :

NP_005517.1

ncbi gb acc num :

NM_005526.2

uniprot acc num :

Q00613

ncbi mol weight :

57260

ncbi summary :

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; cytosol. Molecular Function: protein binding; chromatin binding; transcription factor activity. Biological Process: negative regulation of cell proliferation; embryonic placenta development; embryonic process involved in female pregnancy; mRNA transcription; female meiosis; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; defense response; response to lipopolysaccharide; positive regulation of multicellular organism growth; negative regulation of tumor necrosis factor production; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage