PI4K2A Antibody (C-term) | MyBioSource MBS9211629 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PI4K2A Antibody (C-term)

catalog :

MBS9211629

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

PI4K2A Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PI4K2A antibody detected the PI4K2A protein (arrow).

image 2 :

All lanes : Anti-PI4K2A Antibody (C-term) at1:2000 dilution Lane 1: HepG2 whole cell lysateLane 2: Hela whole cell lysate Lane 3: HL-60whole cell lysate Lysates/proteins at 20 ?g perlane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 54 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.

product information

catalog number :

MBS9211629

products type :

Antibody

products full name :

PI4K2A Antibody (C-term)

products short name :

[PI4K2A]

products name syn :

[Phosphatidylinositol 4-kinase type 2-alpha; Phosphatidylinositol 4-kinase type II-alpha; PI4K2A]

other names :

[phosphatidylinositol 4-kinase type 2-alpha; Phosphatidylinositol 4-kinase type 2-alpha; phosphatidylinositol 4-kinase type 2-alpha; phosphatidylinositol 4-kinase type 2 alpha; Phosphatidylinositol 4-kinase type II-alpha]

products gene name :

[PI4K2A]

other gene names :

[PI4K2A; PI4K2A; PI4KII; PIK42A]

uniprot entry name :

P4K2A_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Zebrafish, Rat)

sequence positions :

[387-415]

sequence length :

479

specificity :

This PI4K2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human PI4K2A.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

other info1 :

Function: Membrane-bound phosphatidylinositol-4kinase (PI4- kinase) that catalyzes thephosphorylation of phosphatidylinositol (PI) tophosphatidylinositol 4-phosphate (PI4P), alipid that plays important roles inendocytosis, Golgi function, protein sortingand membrane trafficking and is required forprolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) tophosphatidylinositol 4-phosphate (PI4P) is thefirst committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2),a precursor of the second messenger inositol1,4,5-trisphosphate (InsP3). Cellular Location: Golgi apparatus, trans-Golgi networkmembrane; Lipid-anchor. Membrane raft. Cellprojection, dendrite{ECO:0000250 UniProtKB:Q2TBE6}. Celljunction, synapse, presynaptic cell membrane{ECO:0000250 UniProtKB:Q2TBE6}. Celljunction, synapse, synaptosome{ECO:0000250 UniProtKB:Q2TBE6}Mitochondrion{ECO:0000250 UniProtKB:Q2TBE6}.Endosome Cytoplasmic vesicle. Membrane;Lipid-anchor. Cell membrane Note=Localizedin neuronal cell body. Transported fromneuronal cell body to neuron projections in aBLOC-1- and AP-3-complexes- dependentmanner. Enriched in neurite tips and neuronprojections in a BLOC-1- andAP-3-complexes-dependent manner (Bysimilarity) Found in subdomains of the plasma membrane termed non-caveolar membranerafts. {ECO:0000250 UniProtKB:Q2TBE6,ECO:0000250 UniProtKB:Q99M64. Tissue location: Widely expressed. Highest expression is observed in kidney, brain, heart, skeletalmuscle, and placenta and lowest expressionis observed in colon, thymus, and small intestine.

products categories :

Signal Transduction

products description :

Phosphatidylinositolpolyphosphates (PtdInsPs) are. centrally involved in many biologic processes, ranging from cell. growth and organization of the actin cytoskeleton to endo- and. exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an. essential step in the biosynthesis of PtdInsPs (Barylko et al.,. 2001 [PubMed 11244087]).

products references :

Li, J., et al. Oncogene 29(17):2550-2559(2010). Qin, Y., et al. J. Biol. Chem. 284(34):22544-22548(2009). Salazar, G., et al. J. Biol. Chem. 284(3):1790-1802(2009). Craige, B., et al. Mol. Biol. Cell 19(4):1415-1426(2008). Olsen, J.V., et al. Cell 127(3):635-648(2006)

ncbi gi num :

13559514

ncbi acc num :

NP_060895.1

ncbi gb acc num :

NM_018425.3

uniprot acc num :

Q9BTU6

ncbi mol weight :

54022

ncbi summary :

Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008]

uniprot summary :

PI4K2A: Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells. Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily. Protein type: EC 2.7.1.67; Kinase, lipid; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q24. Cellular Component: Golgi apparatus; neuron projection; mitochondrion; synaptic vesicle membrane; lysosomal membrane; integral to plasma membrane; dendrite; perikaryon; cytosol; lipid raft; presynaptic membrane; cell soma; membrane; early endosome membrane; intrinsic to membrane; cytoplasmic vesicle; cell junction; endosome. Molecular Function: 1-phosphatidylinositol 4-kinase activity; protein binding; magnesium ion binding; ATP binding. Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; basophil degranulation; phosphatidylinositol biosynthetic process

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage