NETO2 Antibody (N-term) | MyBioSource MBS9211489 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NETO2 Antibody (N-term)

catalog :

MBS9211489

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Overlay histogram showing Jurkat cells stained with MBS9211489 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9211489, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image 2 :

Anti-NETO2 Antibody (N-term ) at 1:2000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

NETO2 Antibody (N-term ) (Cat. #MBS9211489)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NETO2 Antibody (N-term ) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9211489

products type :

Antibody

products full name :

NETO2 Antibody (N-term)

products short name :

[NETO2]

products name syn :

[Neuropilin and tolloid-like protein 2; Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2; NETO2; BTCL2]

other names :

[neuropilin and tolloid-like protein 2 isoform 2; Neuropilin and tolloid-like protein 2; neuropilin and tolloid-like protein 2; neuropilin (NRP) and tolloid (TLL)-like 2; Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2]

products gene name :

[NETO2]

other gene names :

[NETO2; NETO2; BTCL2; NEOT2; BTCL2]

uniprot entry name :

NETO2_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Mouse, Rat)

sequence positions :

[174-203]

specificity :

This NETO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the N-terminal region of human NETO2.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. FC~~1:25. IHC-P~~1:10~50

image1 heading :

Flow Cytometry

image2 heading :

Western Blot

image3 heading :

Immunohistochemistry

other info1 :

Function: Accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3. Increases kainate-receptor channel activity, slowing the decay kinetics of the receptors, without affecting their expression at the cell surface, and increasing the open probability of the receptor channels. Modulates the agonist sensitivity of kainate receptors. Slows the decay of kainate receptor-mediated excitatory postsynaptic currents (EPSCs), thus directly influencing synaptic transmission (By similarity).

other info2 :

Cellular Location: Membrane; Single-pass type I membrane protein

products categories :

Neuroscience

products description :

This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. It also has an intracellular FXNPXY-like motif, which has been shown in other proteins to be essential for the internalization of clathrin coated pits during endocytosis. Alternatively spliced transcript variants have been observed, but they have not been fully characterized. [provided by RefSeq].

products references :

Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Stohr, H., et al. Gene 286(2):223-231(2002)

ncbi gi num :

319996652

ncbi acc num :

NP_001188406.1

ncbi gb acc num :

NM_001201477.1

uniprot acc num :

Q8NC67

ncbi mol weight :

59393

ncbi summary :

This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. A similar gene in rats encodes a protein that modulates glutamate signaling in the brain by regulating kainate receptor function. Expression of this gene may be a biomarker for proliferating infantile hemangiomas. A pseudogene of this gene is located on the long arm of chromosome 8. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage