Glypican-1 Antibody (N-term) | MyBioSource MBS9211418 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Glypican-1 Antibody (N-term)

catalog :

MBS9211418

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Glypican-1 Antibody (N-term) in Hela cell line lysates (35ug/lane). Glypican-1 (arrow) was detected using the purified Pab.

image 2 :

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with Glypican-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 3 :

Glypican-1 Antibody (N-term) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9211418

products type :

Antibody

products full name :

Glypican-1 Antibody (N-term)

products short name :

[Glypican-1]

products name syn :

[Glypican-1; Secreted glypican-1; GPC1]

other names :

[glypican-1; Glypican-1; glypican-1; glypican 1]

products gene name :

[GPC1]

other gene names :

[GPC1; GPC1; glypican]

uniprot entry name :

GPC1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine, Mouse)

sequence positions :

[6-31]

sequence length :

558

specificity :

This Glypican-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-31 amino acids from the N-terminal region of human Glypican-1.

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P, FC- E

app notes :

WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info2 :

Function: Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Cellular location : Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Endosome. Note=S-nitrosylated form recycled in endosomes. Localizes to CAV1-containing vesicles close to the cell surface. Cleavage of heparan sulfate side chains takes place mainly in late endosomes. Associates with both forms of PRNP in lipid rafts. Colocalizes with APP in perinuclear compartments and with CP in intracellular compartments. Associates with fibrillar APP Abeta peptides in lipid rafts in Alzheimer disease brains

products categories :

Cancer; Neuroscience; Signal Transduction

products description :

GPC1(Glypican 1) is a cell surface proteoglycan that contains heparan sulphate. The protein is attached to the cell membrane by a GPI anchor. GPC1 is required for efficient TGF-beta1 signalling in pancreatic cancer cells. Members of the glypican-related integral membrane proteoglycan family (GRIPS) may play a role in the control of cell division and growth regulation.

products references :

Sjoeblom T., et.al., Science 314:268-274(2006).

ncbi gi num :

167001141

ncbi acc num :

NP_002072.2

ncbi gb acc num :

NM_002081.2

uniprot acc num :

P35052

ncbi summary :

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]

uniprot summary :

GPC1: Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Associates (via the heparan sulfate side chains) with fibrillar APP-beta amyloid peptides in primitive and classic amyloid plaques and may be involved in the deposition of these senile plaques in the Alzheimer disease (AD) brain. Misprocessing of GPC1 is found in fibroblasts of patients with Niemann-Pick Type C1 disease. This is due to the defective deaminative degradation of heparan sulfate chains. Belongs to the glypican family. Protein type: Membrane protein, GPI anchor; Extracellular matrix; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q35-q37. Cellular Component: extracellular space; proteinaceous extracellular matrix; lysosomal lumen; integral to plasma membrane; Golgi lumen; plasma membrane; endosome; lipid raft. Molecular Function: heparan sulfate proteoglycan binding; copper ion binding; fibroblast growth factor binding; laminin binding. Biological Process: axon guidance; phototransduction, visible light; heparan sulfate proteoglycan catabolic process; glycosaminoglycan metabolic process; negative regulation of fibroblast growth factor receptor signaling pathway; pathogenesis; Schwann cell differentiation; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; myelin formation; glycosaminoglycan catabolic process; carbohydrate metabolic process; retinoid metabolic process

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage