EN1 (Engrailed 1) Antibody (N-term) | MyBioSource MBS9211211 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EN1 (Engrailed 1) Antibody (N-term)

catalog :

MBS9211211

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-EN1 Antibody (N-term) at 1:2000 dilution. Lane 1: U-2OS whole cell lysate . Lane 2: U87-MG whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: 293T/17 whole cell lysate. Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

All lanes : Anti-EN1 Antibody (N-term) at 1:1000 dilution Lane 1: U-2OS whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

All lanes : Anti-EN1 Antibody (N-term) at 1:1000 dilution Lane 1: human fetal brain lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9211211

products type :

Antibody

products full name :

EN1 (Engrailed 1) Antibody (N-term)

products short name :

[EN1 (Engrailed 1)]

products name syn :

[Homeobox protein engrailed-1; Homeobox protein en-1; Hu-En-1; EN1]

other names :

[homeobox protein engrailed-1; Homeobox protein engrailed-1; homeobox protein engrailed-1; engrailed homeobox 1]

products gene name :

[EN1]

other gene names :

[EN1; EN1; Homeobox protein en-1; Hu-En-1]

uniprot entry name :

HME1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[1-30]

sequence length :

392

specificity :

This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB~~1:1000. IF~~1:10~50. IHC-P~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot

image3 heading :

Western Blot

image4 heading :

Immunofluorescent

image4 description :

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (Human Sarcoma cell line) cells labeling Pdx1 with MBS9211211 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Immunofluorescence image showing nucleus and nucleoli staining on U-2 OS cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).

image5 heading :

Immunohistochemistry

image5 description :

MBS9211211 staining EN1 in Human tonsil tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

other info1 :

Antigen Type: Synthetic Peptide. Crown Antibody: Yes

other info2 :

Antigen Source: HUMAN. Clone Names: RB13885

products categories :

Crown Antibodies; Developmental Biology; Neuroscience; Signal Transduction

products description :

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

products references :

Bachar-Dahan,L., Mol. Biol. Cell 17 (6), 2572-2580 (2006). Kohler,A., Genomics 15 (1), 233-235 (1993)

ncbi gi num :

126090909

ncbi acc num :

NP_001417.3

ncbi gb acc num :

NM_001426.3

uniprot acc num :

Q05925

ncbi mol weight :

40115

ncbi summary :

uniprot summary :

EN1: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the engrailed (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]. Protein type: Cell development/differentiation; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 2q14.2. Cellular Component: membrane; nucleus. Biological Process: embryonic forelimb morphogenesis; anatomical structure morphogenesis; dorsal/ventral pattern formation; pigmentation; multicellular organism growth; midbrain development; midbrain-hindbrain boundary development; neuron development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; skeletal development; proximal/distal pattern formation; hindbrain development

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage