Human Apolipoprotein C3, apo-C3 ELISA Kit | MyBioSource MBS921095 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Apolipoprotein C3, apo-C3 ELISA Kit

catalog :

MBS921095

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS921095

products type :

ELISA Kit

products full name :

Human Apolipoprotein C3, apo-C3 ELISA Kit

products short name :

apolipoprotein C-III

products name syn :

Human Apolipoprotein C3; apo-C3ELISA Kit; APOCIII; MGC150353; ; apolipoprotein C-III

other names :

apolipoprotein C-III; Apolipoprotein C-III; apolipoprotein C-III; apo-CIII; apoC-III; apolipoprotein C3; apolipoprotein C-III; Apolipoprotein C3

products gene name :

APOC3

other gene names :

APOC3; APOC3; HALP2; APOCIII; Apo-CIII; ApoC-III

uniprot entry name :

APOC3_HUMAN

reactivity :

Human

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of Human APOC3. No significant cross-reactivity or interference between Human APOC3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 15.6 ng/ml-1000 ng/ml. Sensitivity: 3.9 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Lipid degradation

products description :

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with apo-C3. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for apo-C3. The competitive inhibition reaction is launched between with pre-coated apo-C3 and apo-C3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of apo-C3 in the samples. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4557323

ncbi acc num :

NP_000031.1

ncbi gb acc num :

NM_000040.1

uniprot acc num :

P02656

ncbi mol weight :

10,852 Da

ncbi pathways :

Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

ncbi summary :

Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]

uniprot summary :

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family. Protein type: Secreted; Lipid-binding; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: extracellular space; chylomicron; early endosome; extracellular region. Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity. Biological Process: response to drug; phototransduction, visible light; cholesterol metabolic process; response to peptide hormone stimulus; regulation of Cdc42 protein signal transduction; cholesterol efflux; lipoprotein metabolic process; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; phospholipid efflux; negative regulation of lipid catabolic process; lipoprotein transport; triacylglycerol catabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; triacylglycerol mobilization; negative regulation of lipid metabolic process; inflammatory response; retinoid metabolic process; negative regulation of fatty acid biosynthetic process; response to nutrient. Disease: Apolipoprotein C-iii Deficiency

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage