Human Leukocyte elastase inhibitor, SERPINB1 ELISA Kit | MyBioSource MBS921070 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Leukocyte elastase inhibitor, SERPINB1 ELISA Kit

catalog :

MBS921070

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS921070

products type :

ELISA Kit

products full name :

Human Leukocyte elastase inhibitor, SERPINB1 ELISA Kit

products short name :

serpin peptidase inhibitor, clade B (ovalbumin) , member 1

products name syn :

Human Leukocyte elastase inhibitor (SERPINB1) ELISA kit; EI; ELANH2; LEI; M/NEI; MNEI; PI2; anti-elastase; protease inhibitor 2 (anti-elastase); monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 1; serpin peptidase inhibitor; clade B (ovalbumin); member 1

other names :

leukocyte elastase inhibitor; Leukocyte elastase inhibitor; leukocyte elastase inhibitor; peptidase inhibitor 2; epididymis luminal protein 57; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1; serpin peptidase inhibitor, clade B (ovalbumin), member 1; Monocyte/neutrophil elastase inhibitor; EI; M/NEI; Peptidase inhibitor 2; PI-2; Serpin B1

products gene name :

SERPINB1

other gene names :

SERPINB1; SERPINB1; EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2

uniprot entry name :

ILEU_HUMAN

reactivity :

Human

sequence length :

379

specificity :

This assay has high sensitivity and excellent specificity for detection of human SERPINB1. No significant cross-reactivity or interference between human SERPINB1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 0.6 25 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human SERPINB1 is typically less than 0.156 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

13489087

ncbi acc num :

NP_109591.1

ncbi gb acc num :

NM_030666.3

uniprot acc num :

P30740

ncbi mol weight :

42,742 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191)

ncbi summary :

The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

uniprot summary :

SERPINB1: Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Belongs to the serpin family. Ov-serpin subfamily. Chromosomal Location of Human Ortholog: 6p25. Cellular Component: extracellular space; membrane; cytoplasm. Molecular Function: serine-type endopeptidase inhibitor activity

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage