Reptin/TIP49B/RUVB2 Antibody (C-term) | MyBioSource MBS9210678 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Reptin/TIP49B/RUVB2 Antibody (C-term)

catalog :

MBS9210678

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

The anti-RUVBL21 Pab is used in Western blot to detect RUVBL2 in mouse kidney (left) and SK-Br-3 (right) tissue/cell line lysates.

image 2 :

Western blot analysis of RUVBL2 (arrow) using rabbit polyclonal RUVBL2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL2 gene.

product information

catalog number :

MBS9210678

products type :

Antibody

products full name :

Reptin/TIP49B/RUVB2 Antibody (C-term)

products short name :

[Reptin/TIP49B/RUVB2]

products name syn :

[RuvB-like 2; 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; ECP-51; INO80 complex subunit J; Repressing pontin 52; Reptin 52; TIP49b; TIP60-associated protein 54-beta; TAP54-beta; RUVBL2; INO80J; TIP48; TIP49B]

other names :

[ruvB-like 2; RuvB-like 2; ruvB-like 2; RuvB-like AAA ATPase 2; 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; ECP-51; INO80 complex subunit J; Repressing pontin 52; Reptin 52; TIP49b; TIP60-associated protein 54-beta; TAP54-beta]

products gene name :

[RUVBL2]

other gene names :

[RUVBL2; RUVBL2; RVB2; TIH2; ECP51; TIP48; CGI-46; INO80J; REPTIN; TIP49B; INO80J; TIP48; TIP49B; 48 kDa TBP-interacting protein; ECP-51; Reptin 52; TAP54-beta]

uniprot entry name :

RUVB2_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse (Predicted Reactivity: Bovine, Xenopus)

sequence positions :

[400-430]

sequence length :

463

specificity :

This Reptin/TIP49B/RUVB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-430 amino acids from the C-terminal region of human Reptin/TIP49B/RUVB2.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN. Function: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes. Cellular Location: Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. Tissue Location: Ubiquitously expressed. Highly expressed in testis and thymus.

products description :

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.

products references :

Bauer, A., et al., EMBO J. 19(22):6121-6130 (2000). Parfait, B., et al., Ann. Genet. 43(2):69-74 (2000). Wood, M.A., et al., Mol. Cell 5(2):321-330 (2000). Salzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999). Kanemaki, M., et al., J. Biol. Chem. 274(32):22437-22444 (1999).

ncbi gi num :

5730023

ncbi acc num :

NP_006657.1

ncbi gb acc num :

NM_006666.1

uniprot acc num :

Q9Y230

ncbi mol weight :

51157

ncbi summary :

uniprot summary :

RUVBL2: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (5 to 3 ) activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Forms homohexameric rings (Probable). Can form a dodecamer with RUVBL1 made of two stacked hexameric rings; however, even though RUVBL1 and RUVBL2 are present in equimolar ratio, the oligomeric status of each hexamer is not known. Oligomerization may regulate binding to nucleic acids and conversely, binding to nucleic acids may affect the dodecameric assembly. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin- remodeling INO80 complex; specifically part of a complex module associated with the helicase ATP-binding and the helicase C- terminal domain of INO80. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with IGHMBP2. Interacts with TELO2. Ubiquitously expressed. Highly expressed in testis and thymus. Belongs to the RuvB family. Protein type: EC 3.6.4.12; Helicase. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: nucleoplasm; NuA4 histone acetyltransferase complex; nuclear matrix; membrane; cytoplasm; intracellular; ribonucleoprotein complex; nucleus. Molecular Function: ATP-dependent DNA helicase activity; identical protein binding; protein binding; DNA helicase activity; chromatin DNA binding; ATP-dependent 5 -3 DNA helicase activity; ATPase activity; unfolded protein binding; damaged DNA binding; ATP binding. Biological Process: chromatin remodeling; establishment and/or maintenance of chromatin architecture; protein folding; transcription, DNA-dependent; regulation of growth; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; DNA repair; DNA duplex unwinding; DNA recombination

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage