M Sirt3 Antibody (C-term) | MyBioSource MBS9210535 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

M Sirt3 Antibody (C-term)

catalog :

MBS9210535

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-Sirt3 Antibody (C-term) at 1:64000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Anti-Sirt3 Antibody (C-term) at 1:2000 dilution + CHO whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

Anti-Mouse Sirt3 Antibody (C-term) at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST

product information

catalog number :

MBS9210535

products type :

Antibody

products full name :

M Sirt3 Antibody (C-term)

products short name :

[M Sirt3]

products name syn :

[NAD-dependent protein deacetylase sirtuin-3; 351-; Regulatory protein SIR2 homolog 3; SIR2-like protein 3; mSIR2L3; Sirt3; Sir2l3]

other names :

[NAD-dependent protein deacetylase sirtuin-3 isoform 1; NAD-dependent protein deacetylase sirtuin-3; NAD-dependent protein deacetylase sirtuin-3; sirtuin 3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3; mSIR2L3]

products gene name :

[Sirt3]

other gene names :

[Sirt3; Sirt3; Sir2l3; AI848213; 2310003L23Rik; Sir2l3; mSIR2L3]

uniprot entry name :

SIR3_MOUSE

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Mouse, hamster

sequence positions :

[304-334]

sequence length :

257

specificity :

This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)

app notes :

WB~~1:2000. IHC-P~~1:50~100

image1 heading :

Western Blot

image2 heading :

Western Blot

image3 heading :

Western Blot

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Sirt3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

other info2 :

Function: NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. Cellular Location: Isoform L: Mitochondrion matrix. Tissue Location: Strongly expressed in liver and kidney. Weakly expressed in lung.

products categories :

Crown Antibodies; Cancer; Cardiovascular; Cell Biology; Metabolism; Neuroscience; Signal Transduction

products description :

SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

products references :

Hirschey, M.D., et al. Nature 464(7285):121-125(2010). Pillai, V.B., et al. J. Biol. Chem. 285(5):3133-3144(2010). Kim, H.S., et al. Cancer Cell 17(1):41-52(2010)

ncbi gi num :

188219522

ncbi acc num :

NP_001120823.1

ncbi gb acc num :

NM_001127351.1

uniprot acc num :

Q8R104

ncbi mol weight :

36615

ncbi pathways :

, Pathway (64384); , Pathway (64384)

uniprot summary :

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product. Protein type: Deacetylase; Mitochondrial; EC 3.5.1.-. Cellular Component: membrane; mitochondrion; mitochondrial matrix; cytoplasm. Molecular Function: (3,5-dichlorophenylurea)acetate amidohydrolase activity; zinc ion binding; hydrolase activity; metal ion binding; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; N-acetylgalactosamine-6-phosphate deacetylase activity; N2-acetyl-L-lysine deacetylase activity; protein binding; iprodione amidohydrolase activity; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); indoleacetamide hydrolase activity; protein N-terminal asparagine amidohydrolase activity; histone deacetylase activity; O-succinylbenzoate synthase activity; NAD binding. Biological Process: protein amino acid deacetylation; aerobic respiration; positive regulation of insulin secretion

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage