AGER Antibody (N-term) | MyBioSource MBS9210446 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

AGER Antibody (N-term)

catalog :

MBS9210446

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of AGER Antibody (N-term) in mouse lung tissue lysates (35ug/lane). AGER (arrow) was detected using the purified Pab.

image 2 :

AGER Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGER Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

image 3 :

AGER Antibody (N-term) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9210446

products type :

Antibody

products full name :

AGER Antibody (N-term)

products short name :

[AGER]

products name syn :

[Advanced glycosylation end product-specific receptor; Receptor for advanced glycosylation end products; AGER; RAGE]

other names :

[advanced glycosylation end product-specific receptor isoform 1; Advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor; Receptor for advanced glycosylation end products]

products gene name :

[AGER]

other gene names :

[AGER; AGER; RAGE; RAGE]

uniprot entry name :

RAGE_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, Mouse

sequence positions :

[24-52]

specificity :

This AGER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-52 amino acids from the N-terminal region of human AGER.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

0.5 mg/ml

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. IHC-P~~1:10~50. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Function: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.

other info2 :

Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 10: Cell membrane; Single-pass type I membrane protein. Tissue Location: Endothelial cells

products categories :

Cardiovascular; Developmental Biology; Neuroscience

products description :

AGER encodes a member of the immunoglobulin superfamily of cell surface molecules. It is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. The gene lies within the major histocompatibility complex (MHC) class III region on chromosome 6.

products references :

Rojas, A., et al. Carcinogenesis 31(3):334-341(2010). Engelen, L., et al. J. Hypertens. 28(2):285-293(2010). Poon, P.Y., et al. Clin. Nephrol. 73(1):44-50(2010). Hancock, D.B., et al. Nat. Genet. 42(1):45-52(2010)

ncbi gi num :

10835203

ncbi acc num :

NP_001127.1

ncbi gb acc num :

NM_001136.4

uniprot acc num :

Q15109

ncbi mol weight :

42803

ncbi pathways :

Formaldehyde Oxidation II (glutathione-dependent) Pathway (177); Formaldehyde Oxidation II (glutathione-dependent) Pathway (177); Formaldehyde Oxidation II (glutathione-dependent) Pathway (177); Formaldehyde Oxidation II (glutathione-dependent) Pathway (177); Formaldehyde Oxidation II (glutathione-dependent) Pathway (177); Formaldehyde Oxidation II (glutathione-dependent) Pathway (177); Formaldehyde Oxidation II (glutathione-dependent) Pathway (177); Formaldehyde Oxidation II (glutathione-dependent) Pathway (177); Formaldehyde Oxidation II (glutathione-dependent) Pathway (177); Formaldehyde Oxidation II (glutathione-dependent) Pathway (177)

ncbi summary :

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

uniprot summary :

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell cycle regulation; Motility/polarity/chemotaxis; Receptor, misc. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: integral to plasma membrane; plasma membrane; extracellular region. Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; receptor activity. Biological Process: cell surface receptor linked signal transduction; response to wounding; innate immune response; inflammatory response; neurite development; activation of NF-kappaB transcription factor; induction of positive chemotaxis

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage