CXCR3 Antibody (Center) | MyBioSource MBS9210391 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CXCR3 Antibody (Center)

catalog :

MBS9210391

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

CXCR3 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CXCR3 antibody detected the CXCR3 protein (arrow).

image 2 :

CXCR3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CXCR3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

image 3 :

CXCR3 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9210391

products type :

Antibody

products full name :

CXCR3 Antibody (Center)

products short name :

[CXCR3]

products name syn :

[C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; CKR-L2; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; CD183; CXCR3; GPR9]

other names :

[C-X-C chemokine receptor type 3 isoform 2; C-X-C chemokine receptor type 3; C-X-C chemokine receptor type 3; chemokine (C-X-C motif) receptor 3; CKR-L2; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; CD_antigen: CD183]

products gene name :

[CXCR3]

other gene names :

[CXCR3; CXCR3; GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R; GPR9; CXC-R3; CXCR-3; IP-10 receptor]

uniprot entry name :

CXCR3_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[140-167]

sequence length :

415

specificity :

This CXCR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-167 amino acids from the Central region of human CXCR3.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Antigen Type: Synthetic Peptide. Clone Names: RB23384

other info2 :

Antigen Source: HUMAN. Function: Isoform 1: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed: 12782716 ). Binds to CCL21. Probably promotes cell chemotaxis response. Isoform 3: Mediates the activity of CXCL11. Cellular Location: Isoform 1: Cell membrane; Multi-pass membrane protein. Tissue Location: Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level)

products categories :

Cardiovascular; Immunology; Signal Transduction; Phospho Antibodies

products description :

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells. Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases. Multiple transcript variants encoding different isoforms have been found for this gene.

products references :

Zhou, J., et al. J. Exp. Med. 207(9):1951-1966(2010). Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010). Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010). Miekus, K., et al. Folia Histochem. Cytobiol. 48(1):104-111(2010). Ohri, C.M., et al. BMC Cancer 10, 172 (2010) :

ncbi gi num :

218563730

ncbi acc num :

NP_001136269.1

ncbi gb acc num :

NM_001142797.1

uniprot acc num :

P49682

ncbi mol weight :

40660

ncbi pathways :

Fructose And Mannose Metabolism Pathway (2833); Fructose And Mannose Metabolism Pathway (2833); Fructose And Mannose Metabolism Pathway (2833); Fructose And Mannose Metabolism Pathway (2833); Fructose And Mannose Metabolism Pathway (2833); Fructose And Mannose Metabolism Pathway (2833); Fructose And Mannose Metabolism Pathway (2833); Fructose And Mannose Metabolism Pathway (2833); Fructose And Mannose Metabolism Pathway (2833); Fructose And Mannose Metabolism Pathway (2833)

ncbi summary :

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]

uniprot summary :

CXCR3: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1. Chromosomal Location of Human Ortholog: Xq13. Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane. Molecular Function: C-X-C chemokine binding; chemokine receptor activity; chemokine binding; receptor activity; C-X-C chemokine receptor activity. Biological Process: apoptosis; positive regulation of chemotaxis; calcium-mediated signaling; positive regulation of cAMP metabolic process; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; negative regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of leukocyte migration; negative regulation of endothelial cell proliferation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of release of sequestered calcium ion into cytosol; angiogenesis; cell motility; inflammatory response; cell adhesion

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage