WNT3A Antibody (Center) | MyBioSource MBS9210359 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

WNT3A Antibody (Center)

catalog :

MBS9210359

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

WNT3A Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the WNT3A antibody detected the WNT3A protein (arrow).

image 2 :

WNT3A Antibody(Center)(MBS9210359)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WNT3A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9210359

products type :

Antibody

products full name :

WNT3A Antibody (Center)

products short name :

[WNT3A]

products name syn :

[Protein Wnt-3a; WNT3A]

other names :

[protein Wnt-3a; Protein Wnt-3a; protein Wnt-3a; wingless-type MMTV integration site family, member 3A]

products gene name :

[WNT3A]

other gene names :

[WNT3A; WNT3A]

uniprot entry name :

WNT3A_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Chicken, Mouse, Xenopus)

sequence positions :

[151-180]

sequence length :

352

specificity :

This WNT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the Central region of human WNT3A.

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P, E

app notes :

WB~~1:1000. IHC-P~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info2 :

Function: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Cellular location : Secreted, extracellular space, extracellular matrix. Tissue location: Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate

products categories :

Developmental Biology; Neuroscience; Signal Transduction

products description :

The WNT gene family consists of structurally related genes. which encode secreted signaling proteins. These proteins have been. implicated in oncogenesis and in several developmental processes,. including regulation of cell fate and patterning during. embryogenesis. This gene is a member of the WNT gene family. It. encodes a protein which shows 96% amino acid identity to mouse. Wnt3A protein, and 84% to human WNT3 protein, another WNT gene. product. This gene is clustered with WNT14 gene, another family. member, in chromosome 1q42 region.

products references :

Sengupta, P.K., et al. J. Biol. Chem. 285(41):31164-31173(2010). Hubner, R., et al. Biochem. Biophys. Res. Commun. 400(3):358-362(2010). von Marschall, Z., et al. Biochem. Biophys. Res. Commun. 400(3):299-304(2010). Shin, H., et al. J. Dermatol. Sci. 58(2):91-96(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :

ncbi gi num :

14916475

ncbi acc num :

NP_149122.1

ncbi gb acc num :

NM_033131.3

uniprot acc num :

P56704

ncbi pathways :

Glycolysis / Gluconeogenesis Pathway (89780); Glycolysis / Gluconeogenesis Pathway (89780); Glycolysis / Gluconeogenesis Pathway (89780); Glycolysis / Gluconeogenesis Pathway (89780); Glycolysis / Gluconeogenesis Pathway (89780); Glycolysis / Gluconeogenesis Pathway (89780); Glycolysis / Gluconeogenesis Pathway (89780); Glycolysis / Gluconeogenesis Pathway (89780); Glycolysis / Gluconeogenesis Pathway (89780); Glycolysis / Gluconeogenesis Pathway (89780)

ncbi summary :

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

uniprot summary :

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q42. Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; early endosome membrane; Golgi lumen; endoplasmic reticulum lumen; extracellular region; plasma membrane. Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding. Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; cell proliferation in forebrain; positive regulation of receptor internalization; positive regulation of caspase activity; positive regulation of collateral sprouting in the absence of injury; palate development; Wnt receptor signaling pathway through beta-catenin; negative regulation of axon extension involved in axon guidance; negative regulation of neurogenesis; Wnt receptor signaling pathway in forebrain neuroblast division; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage