ASAH2 Antibody (C-term) | MyBioSource MBS9210331 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ASAH2 Antibody (C-term)

catalog :

MBS9210331

quantity :

0.08 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9210331

products type :

Antibody

products full name :

ASAH2 Antibody (C-term)

products short name :

ASAH2

products name syn :

Neutral ceramidase; N-CDase; NCDase; Acylsphingosine deacylase 2; BCDase; LCDase; hCD; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase; Neutral ceramidase soluble form; ASAH2; HNAC1

other names :

neutral ceramidase isoform b; Neutral ceramidase; neutral ceramidase; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; Acylsphingosine deacylase 2; BCDase; LCDase; hCD

products gene name :

ASAH2

other gene names :

ASAH2; ASAH2; HNAC1; BCDase; LCDase; NCDase; N-CDase; HNAC1; N-CDase; NCDase; hCD

uniprot entry name :

ASAH2_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

734-762

sequence length :

745

specificity :

This ASAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 734-762 amino acids from the C-terminal region of human ASAH2.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN. Function: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Cellular Location: Cell membrane; Single-pass type II membrane protein. Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N- terminus, which may explain this localization which probably does not exist in vivo. Tissue Location: Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B.

products categories :

Metabolism; Signal Transduction

products description :

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze. hydrolysis of the N-acyl linkage of ceramide, a second messenger in. a variety of cellular events, to produce sphingosine. Sphingosine. exerts both mitogenic and apoptosis-inducing activities, and its. phosphorylated form functions as an intra- and intercellular second. messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed. 11328816]).

products references :

Uchida, Y., et al. J. Invest. Dermatol. 130(10):2472-2480(2010). Hong, K.K., et al. J. Korean Med. Sci. 22(5):862-867(2007). Ohlsson, L., et al. Biochimie 89(8):950-960(2007). Avramopoulos, D., et al. Neurogenetics 8(2):111-120(2007). Galadari, S., et al. Biochem. J. 393 (PT 3), 687-695 (2006) :

ncbi gi num :

221307475

ncbi acc num :

NP_001137446.1

ncbi gb acc num :

NM_001143974.1

uniprot acc num :

Q9NR71

ncbi mol weight :

85516

ncbi pathways :

Fructose And Mannose Metabolism Pathway (56624); Fructose And Mannose Metabolism Pathway (56624); Fructose And Mannose Metabolism Pathway (56624); Fructose And Mannose Metabolism Pathway (56624); Fructose And Mannose Metabolism Pathway (56624); Fructose And Mannose Metabolism Pathway (56624); Fructose And Mannose Metabolism Pathway (56624); Fructose And Mannose Metabolism Pathway (56624); Fructose And Mannose Metabolism Pathway (56624); Fructose And Mannose Metabolism Pathway (56624)

ncbi summary :

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]

uniprot summary :

ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid Metabolism - sphingolipid; Membrane protein, integral; EC 3.5.1.23; Hydrolase. Chromosomal Location of Human Ortholog: 10q11.21. Cellular Component: mitochondrion; plasma membrane; integral to membrane. Molecular Function: ceramidase activity. Biological Process: sphingolipid metabolic process; apoptosis; glycosphingolipid metabolic process; signal transduction; ceramide metabolic process

size1 :

0.08 mL

price1 :

155 USD

size2 :

0.4 mL

price2 :

340

more info or order :

MyBioSource product webpage