KRAS Antibody (C-term) | MyBioSource MBS9210256 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

KRAS Antibody (C-term)

catalog :

MBS9210256

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

All lanes : Anti-KRAS Antibody (C-term) at 1:500 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293T/17 whole cell lysate. Lane 3: C2C12 whole cell lysate. Lane 4: C6 whole cell lysate. Lane 5: HT-29 whole cell lysate. Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa

image 3 :

All lanes : Anti-KRAS Antibody (C-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: C2C12 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: K562 whole cell lysate Lane 5: PC-12 whole cell lysate. Lane 5: Ramos whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9210256

products type :

Antibody

products full name :

KRAS Antibody (C-term)

products short name :

[KRAS]

products name syn :

[GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; GTPase KRas; N-terminally processed; KRAS; KRAS2; RASK2]

other names :

[GTPase KRas isoform b; GTPase KRas; GTPase KRas; Kirsten rat sarcoma viral oncogene homolog; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras]

products gene name :

[KRAS]

other gene names :

[KRAS; KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS2; RASK2]

uniprot entry name :

RASK_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence positions :

[146-174]

sequence length :

188

specificity :

This KRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the C-terminal region of human KRAS.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

app notes :

WB~~1:2000. IF~~1:10~50. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Immunofluorescence (IF)

image4 description :

Confocal immunofluorescent analysis of KRAS Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

image5 heading :

Flow Cytometry (FC/FACS)

image5 description :

KRAS Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN. Clone Names: RB32708. Function: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed: 23698361 , PubMed: 22711838 ). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer(CRC) cells in a ZNF304-dependent manner(PubMed: 24623306 ). Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol

products categories :

Cancer; Signal Transduction

products description :

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

products references :

Bruckman, K.C., et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110(5):632-637(2010). Wong, K.K., et al. Am. J. Pathol. 177(4):1611-1617(2010). Irahara, N., et al. Diagn. Mol. Pathol. 19(3):157-163(2010). Carotenuto, P., et al. Pharmacogenomics 11(8):1169-1179(2010). Leventopoulos, G., et al. Clin. Exp. Rheumatol. 28(4):556-557(2010)

ncbi gi num :

15718761

ncbi acc num :

NP_004976.2

ncbi gb acc num :

NM_004985.4

uniprot acc num :

P01116

ncbi mol weight :

21656

ncbi pathways :

, Pathway (3845); , Pathway (3845); , Pathway (3845); , Pathway (3845); , Pathway (3845); , Pathway (3845); , Pathway (3845); , Pathway (3845); , Pathway (3845); , Pathway (3845)

ncbi summary :

uniprot summary :

KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Ras; Oncoprotein. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; membrane; mitochondrion; cytoplasm; plasma membrane; cytosol; lipid raft. Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding. Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nerve growth factor receptor signaling pathway; activation of MAPKK activity; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; social behavior; response to mineralocorticoid stimulus; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; striated muscle cell differentiation; innate immune response; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; leukocyte migration. Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Breast Cancer; Lung Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage