NOD1 Antibody (C-term) | MyBioSource MBS9210162 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NOD1 Antibody (C-term)

catalog :

MBS9210162

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-NOD1 Antibody (C-term) at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: NCI-H1299 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: SW480 whole cell lysate Lane 6: Mouse heart lysate Lane 7: Mouse spleen lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST

image 2 :

All lanes : Anti-NOD1 Antibody (C-term) at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: NCI-H1299 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: SW480 whole cell lysate Lane 6: Mouse heart lysate Lane 7: Mouse spleen lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9210162

products type :

Antibody

products full name :

NOD1 Antibody (C-term)

products short name :

[NOD1]

products name syn :

[Nucleotide-binding oligomerization domain-containing protein 1; Caspase recruitment domain-containing protein 4; NOD1; CARD4]

other names :

[nucleotide-binding oligomerization domain-containing protein 1; Nucleotide-binding oligomerization domain-containing protein 1; nucleotide-binding oligomerization domain-containing protein 1; nucleotide-binding oligomerization domain containing 1; Caspase recruitment domain-containing protein 4]

products gene name :

[NOD1]

other gene names :

[NOD1; NOD1; CARD4; NLRC1; CLR7.1; CARD4]

uniprot entry name :

NOD1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[923-951]

sequence length :

953

specificity :

This NOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-951 amino acids from the C-terminal region of human NOD1.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, E

app notes :

WB~~1:1000

image1 heading :

Testing Data (TD)

image2 heading :

Testing Data (TD)

other info2 :

Function: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection. Cellular Location: Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Note=Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells. Tissue Location: Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon

products categories :

Cell Biology

products description :

This gene encodes a member of the NOD (nucleotide-binding. oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a. centrally located nucleotide-binding domain (NBD), and 10 tandem. leucine-rich repeats (LRRs) in its C terminus. The CARD is involved. in apoptotic signaling, LRRs participate in protein-protein. interactions, and mutations in the NBD may affect the process of. oligomerization and subsequent function of the LRR domain. This. protein is an intracellular pattern-recognition receptor (PRR) that. initiates inflammation in response to a subset of bacteria through. the detection of bacterial diaminopimelic acid. Multiple. alternatively spliced transcript variants differring in the 5' UTR. have been described, but the full-length nature of these variants. has not been determined.

products references :

Hutton, M.L., et al. Infect. Immun. 78(11):4523-4531(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Lu, W.G., et al. World J. Gastroenterol. 16(34):4348-4356(2010). Enevold, C., et al. Mult. Scler. 16(8):942-949(2010). Ashton, K.A., et al. BMC Cancer 10, 382 (2010) :

ncbi gi num :

5174617

ncbi acc num :

NP_006083.1

ncbi gb acc num :

NM_006092.2

uniprot acc num :

Q9Y239

ncbi mol weight :

107691

ncbi pathways :

Nicotinate And Nicotinamide Metabolism Pathway (10392); Nicotinate And Nicotinamide Metabolism Pathway (10392); Nicotinate And Nicotinamide Metabolism Pathway (10392); Nicotinate And Nicotinamide Metabolism Pathway (10392); Nicotinate And Nicotinamide Metabolism Pathway (10392); Nicotinate And Nicotinamide Metabolism Pathway (10392); Nicotinate And Nicotinamide Metabolism Pathway (10392); Nicotinate And Nicotinamide Metabolism Pathway (10392); Nicotinate And Nicotinamide Metabolism Pathway (10392); Nicotinate And Nicotinamide Metabolism Pathway (10392)

ncbi summary :

This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Oct 2009]

uniprot summary :

NOD1: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon. Chromosomal Location of Human Ortholog: 7p14.3. Cellular Component: basolateral plasma membrane; apical plasma membrane; cytoplasm; cytosol. Molecular Function: identical protein binding; protein binding; peptidoglycan binding; protein homodimerization activity; CARD domain binding; caspase activator activity; ATP binding. Biological Process: detection of bacterium; apoptosis; activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; stress-activated MAPK cascade; positive regulation of caspase activity; positive regulation of JNK cascade; defense response; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; interleukin-8 biosynthetic process; JNK cascade; detection of biotic stimulus; inflammatory response; toll-like receptor 4 signaling pathway; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage