DLK1 Antibody (C-term) | MyBioSource MBS9210091 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

DLK1 Antibody (C-term)

catalog :

MBS9210091

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-DLK1 Antibody (C-term) at 1:1000 dilution. Lane 1: human placenta lysates. Lane 2: mouse skeletal muscle lysates. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Immunohistochemical analysis of paraffin-embedded H. placenta section using DLK1 Antibody (C-term). MBS9210091 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

image 3 :

Flow cytometric analysis of HepG2 cells using DLK1 Antibody (C-term)(green) compared to an isotype control of mouse IgG2b(blue). MBS9210091 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

product information

catalog number :

MBS9210091

products type :

Antibody

products full name :

DLK1 Antibody (C-term)

products short name :

[DLK1]

products name syn :

[Protein delta homolog 1; DLK-1; pG2; Fetal antigen 1; FA1; DLK1; DLK]

other names :

[protein delta homolog 1; Protein delta homolog 1; protein delta homolog 1; delta-like 1 homolog (Drosophila); pG2]

products gene name :

[DLK1]

other gene names :

[DLK1; DLK1; DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1; DLK; DLK-1; FA1]

uniprot entry name :

DLK1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence length :

383

specificity :

This DLK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 368-402 amino acids from the C-terminal region of human DLK1.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. FC~~1:25. IHC-P~~1:25.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Crown Antibody: Yes

other info2 :

Antigen Source: HUMAN. Function: May have a role in neuroendocrine differentiation. Cellular Location: Membrane; Single-pass type I membrane protein. Tissue Location: Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue

products categories :

Crown Antibodies; Neuroscience; Signal Transduction; Stem Cells

products description :

May have a role in neuroendocrine differentiation.

products references :

Laborda J.,et al.J. Biol. Chem. 268:3817-3820(1993). Lee Y.L.,et al.Biochim. Biophys. Acta 1261:223-232(1995). Helman L.J.,et al.Nucleic Acids Res. 18:685-685(1990). Heilig R.,et al.Nature 421:601-607(2003). Jensen C.H.,et al.Eur. J. Biochem. 225:83-92(1994).

ncbi gi num :

74136023

ncbi acc num :

NP_003827.3

ncbi gb acc num :

NM_003836.5

uniprot acc num :

P80370

ncbi mol weight :

41300

ncbi pathways :

Lysine Biosynthesis Pathway (8788); Lysine Biosynthesis Pathway (8788); Lysine Biosynthesis Pathway (8788); Lysine Biosynthesis Pathway (8788); Lysine Biosynthesis Pathway (8788); Lysine Biosynthesis Pathway (8788); Lysine Biosynthesis Pathway (8788)

ncbi summary :

This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010]

uniprot summary :

DLK1: May have a role in neuroendocrine differentiation. Monomer. Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 14q32. Cellular Component: extracellular space; integral to membrane; external side of plasma membrane. Biological Process: Notch signaling pathway; regulation of gene expression; embryonic skeletal development; multicellular organismal development; negative regulation of Notch signaling pathway; cell differentiation; post-embryonic development

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage