Mouse Pak3 Antibody (N-term) | MyBioSource MBS9209950 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Pak3 Antibody (N-term)

catalog :

MBS9209950

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysates from Hela, Jurkat cell line (from left to right), using Mouse Pak3 Antibody (N-term). MBS9209950 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

image 2 :

Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Pak3 Antibody (N-term). MBS9209950 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

image 3 :

Mouse Pak3 Antibody (N-term) (MBS9209950)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pak3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9209950

products type :

Antibody

products full name :

Mouse Pak3 Antibody (N-term)

products short name :

[Pak3]

products name syn :

[Serine/threonine-protein kinase PAK 3; Beta-PAK; CDC42/RAC effector kinase PAK-B; p21-activated kinase 3; PAK-3; Pak3; Pak-3; Pakb; Stk4]

other names :

[serine/threonine-protein kinase PAK 3 isoform C; Serine/threonine-protein kinase PAK 3; serine/threonine-protein kinase PAK 3; p21 protein (Cdc42/Rac)-activated kinase 3; Beta-PAK; CDC42/RAC effector kinase PAK-B; p21-activated kinase 3; PAK-3]

products gene name :

[Pak3]

other gene names :

[Pak3; Pak3; Stk4; PAK-3; Pak65beta; Pak65alpha; Pak-3; Pakb; Stk4; PAK-3]

uniprot entry name :

PAK3_MOUSE

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Mouse . Predicted Reactivity: Human, Rat, Xenopus

sequence positions :

[124-152]

sequence length :

544

specificity :

This Mouse Pak3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-152 amino acids from the N-terminal region of mouse Pak3.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P,E

app notes :

WB~~1:1000. IHC-P~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Calculated MW: 62398

other info2 :

Function: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. Cellular location: Cytoplasm

products description :

Key regulator of synapse formation and plasticity in the hippocampus.

products references :

Shimogori, T., et al. Nat. Neurosci. 13(6):767-775(2010). Jiang, X.S., et al. Hum. Mol. Genet. 19(7):1347-1357(2010). Demyanenko, G.P., et al. Neuroscience 165(1):107-115(2010). Grimsley-Myers, C.M., et al. J. Neurosci. 29(50):15859-15869(2009). Sansom, S.N., et al. PLoS Genet. 5 (6), E1000511 (2009) :

ncbi gi num :

304307785

ncbi acc num :

NP_001181977.1

ncbi gb acc num :

NM_001195048.1

uniprot acc num :

Q61036

ncbi pathways :

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uniprot summary :

PAK3: a protein kinase of the STE20 family that regulates synapse formation and plasticity in the hippocampus. May be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Forms an activated complex with GTP-bound P21, CDC2 and RAC1 proteins. Missense and truncation mutations linked to nonsyndromic mental retardation type 30 (MRX30). Two alternatively spliced human isoforms have been reported. Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE20 family; PAKA subfamily. Cellular Component: cytoplasm; endosome. Molecular Function: Rho GTPase binding; metal ion binding; nucleotide binding; protein kinase activity; transferase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; GTPase binding; protein binding; transferase activity, transferring phosphorus-containing groups; kinase activity; SH3 domain binding; catalytic activity; ATP binding; receptor signaling protein serine/threonine kinase activity. Biological Process: regulation of actin filament polymerization; axonogenesis; activation of MAPK activity; metabolic process; multicellular organismal development; positive regulation of neuron apoptosis; MAPKKK cascade; dendrite development; response to stress; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation; phosphorylation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage