PLCZ1 Antibody (N-term) | MyBioSource MBS9209842 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PLCZ1 Antibody (N-term)

catalog :

MBS9209842

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of PLCZ1 Antibody (N-term) in CEM, 293 cell line and mouse heart,spleen tissue lysates (35ug/lane). PLCZ1 (arrow) was detected using the purified Pab.

image 2 :

Anti-PLCZ1 Antibody (N-term) at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST

product information

catalog number :

MBS9209842

products type :

Antibody

products full name :

PLCZ1 Antibody (N-term)

products short name :

[PLCZ1]

products name syn :

[1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1; Phosphoinositide phospholipase C-zeta-1; Phospholipase C-zeta-1; PLC-zeta-1; Testis-development protein NYD-SP27; PLCZ1 HGNC:19218)]

other names :

[1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1; phospholipase C, zeta 1; Phosphoinositide phospholipase C-zeta-1; Phospholipase C-zeta-1]

products gene name :

[PLCZ1]

other gene names :

[PLCZ1; PLCZ1; PLCzeta; NYD-SP27; PLC-zeta-1; PLC-zeta-1]

uniprot entry name :

PLCZ1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[26-54]

sequence length :

504

specificity :

This PLCZ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human PLCZ1.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000

image1 heading :

Western Blot (WB)

image2 heading :

Testing Data(TD)

other info1 :

Calculated MW: 70411

other info2 :

Function: The production of the second messenger molecules diacylglycerol (DAG) and inositol1,4,5-trisphosphate (IP3) is mediated byactivated phosphatidylinositol-specificphospholipase C enzymes. In vitro,hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellularCa(2+) oscillations in oocytes solely during Mphase and is involved in inducing oocyteactivation and initiating embryonicdevelopment up to the blastocyst stage. Is there fore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the eggcytoplasm following gamete membrane fusion. May exert an inhibitory effect onphospholipase-C-coupled processes that depend on calcium ions and protein kinase C,including CFTR trafficking and function. Cellular Location: Nucleus {ECO:0000250 UniProtKB:Q8K4D7}.Cytoplasm, perinuclear region{ECO:0000250 UniProtKB:Q8K4D7}Note=Exhibits alternativecytoplasmic/nuclear localization duringdevelopment. Translocates from thepronucleus into cytoplasm upon nuclearenvelope breakdown for mitosis and localizesagain to the pronucleus at interphasefollowing meiosis and mitosis (By similarity).{ECO:0000250 UniProtKB:Q8K4D7. Tissue Location: Expressed specifically in testis and sperm.Weakly expressed in pancreatic-duct cells.Up-regulated in pancreatic-duct cells frompatients with cystic fibros.

products categories :

Cancer; Metabolism

products references :

Yoon, S.Y., et al. J. Clin. Invest. 118(11):3671-3681(2008). Ito, M., et al. Biol. Reprod. 78(6):1081-1090(2008). Yu, Y., et al. Hum. Reprod. 23(2):365-373(2008). Yoshida, N., et al. Development 134(21):3941-3952(2007). Zhu, J.X., et al. Cell Biol. Int. 31(5):521-525(2007)

ncbi gi num :

25188201

ncbi acc num :

NP_149114.2

ncbi gb acc num :

NM_033123.3

uniprot acc num :

Q86YW0

ncbi pathways :

RNA Polymerase Pathway (89869); RNA Polymerase Pathway (89869); RNA Polymerase Pathway (89869); RNA Polymerase Pathway (89869); RNA Polymerase Pathway (89869); RNA Polymerase Pathway (89869); RNA Polymerase Pathway (89869); RNA Polymerase Pathway (89869); RNA Polymerase Pathway (89869); RNA Polymerase Pathway (89869)

ncbi summary :

The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

uniprot summary :

PLCZ1: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)- dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg- activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate; Phospholipase. Chromosomal Location of Human Ortholog: 12p12.3. Cellular Component: perinuclear region of cytoplasm; cytosol; nucleus. Molecular Function: signal transducer activity; calcium ion binding; phosphoinositide phospholipase C activity. Biological Process: inositol phosphate metabolic process; multicellular organismal development; calcium ion transport; egg activation; lipid catabolic process

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage