P4HA1 Antibody (C-term) | MyBioSource MBS9209736 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

P4HA1 Antibody (C-term)

catalog :

MBS9209736

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, cow

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

P4HA1 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the P4HA1 antibody detected the P4HA1 protein (arrow).

product information

catalog number :

MBS9209736

products type :

Antibody

products full name :

P4HA1 Antibody (C-term)

products short name :

[P4HA1]

products name syn :

[Prolyl 4-hydroxylase subunit alpha-1; 4-PH alpha-1; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; P4HA1; P4HA]

other names :

[prolyl 4-hydroxylase subunit alpha-1 isoform 1; Prolyl 4-hydroxylase subunit alpha-1; prolyl 4-hydroxylase subunit alpha-1; prolyl 4-hydroxylase, alpha polypeptide I; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1]

products gene name :

[P4HA1]

other gene names :

[P4HA1; P4HA1; P4HA; P4HA; 4-PH alpha-1]

uniprot entry name :

P4HA1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine)

sequence positions :

[414-442]

sequence length :

516

specificity :

This P4HA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human P4HA1.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000

image1 heading :

Western Blot (WB)

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN. Other Acession: Q1RMU3. Function : Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Cellular Location: Endoplasmic reticulum lumen.

products categories :

Signal Transduction

products description :

This gene encodes a component of prolyl 4-hydroxylase, a. key enzyme in collagen synthesis composed of two identical alpha. subunits and two beta subunits. The encoded protein is one of. several different types of alpha subunits and provides the major. part of the catalytic site of the active enzyme. In collagen and. related proteins, prolyl 4-hydroxylase catalyzes the formation of. 4-hydroxyproline that is essential to the proper three-dimensional. folding of newly synthesized procollagen chains. Alternatively. spliced transcript variants encoding different isoforms have been. described.

products references :

Rose, J. Phd, et al. Mol. Med. (2010) In press :. Gorres, K.L., et al. PLoS ONE 4 (11), E7635 (2009) :. Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006). Fahling, M., et al. J. Biol. Chem. 281(36):26089-26101(2006). Grimmer, C., et al. Am. J. Pathol. 169(2):491-502(2006)

ncbi gi num :

63252886

ncbi acc num :

NP_000908.2

ncbi gb acc num :

NM_000917.3

uniprot acc num :

P13674

ncbi mol weight :

61049

ncbi summary :

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

P4HA1: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - arginine and proline; Mitochondrial; EC 1.14.11.2; Endoplasmic reticulum; Oxidoreductase. Chromosomal Location of Human Ortholog: 10q22.1. Cellular Component: mitochondrion; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; endoplasmic reticulum lumen. Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; procollagen-proline 4-dioxygenase activity; L-ascorbic acid binding; iron ion binding. Biological Process: collagen fibril organization; peptidyl-proline hydroxylation to 4-hydroxy-L-proline

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage