PHB Antibody (N-term) | MyBioSource MBS9209503 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PHB Antibody (N-term)

catalog :

MBS9209503

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysate from A431 cell line, using PHB Antibody (N-term). MBS9209503 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

product information

catalog number :

MBS9209503

products type :

Antibody

products full name :

PHB Antibody (N-term)

products short name :

[PHB]

products name syn :

[Prohibitin; PHB]

other names :

[prohibitin isoform 1; Prohibitin; prohibitin; prohibitin]

products gene name :

[PHB]

other gene names :

[PHB; PHB; PHB1; HEL-215; HEL-S-54e]

uniprot entry name :

PHB_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Drosophila, Bovine, Mouse, Rat)

specificity :

This PHB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 73-107 amino acids from the N-terminal region of human PHB.

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000

image1 heading :

Western Blot (WB)

other info1 :

Cellular Location: Mitochondrion inner membrane. Tissue Location: Widely expressed in different tissues.

products categories :

Crown Antibodies; Cell Biology; Metabolism; Microbiology

products description :

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

products references :

Sato T.,et al.Cancer Res. 52:1643-1646(1992). Sato T.,et al.Genomics 17:762-764(1993). Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. Bienvenut W.V.,et al.Submitted (MAR-2005) to UniProtKB. Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.

ncbi gi num :

527498279

ncbi acc num :

NP_001268425.1

ncbi gb acc num :

NM_001281496.1

uniprot acc num :

P35232

ncbi summary :

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

uniprot summary :

PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer. Protein type: Oncoprotein; Mitochondrial; Transcription, coactivator/corepressor; Chaperone; Nuclear receptor co-regulator; Cell cycle regulation; Apoptosis; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: nucleoplasm; mitochondrion; membrane; integral to plasma membrane; mitochondrial inner membrane; cytoplasm; nucleus. Molecular Function: protein binding; enzyme binding; histone deacetylase binding. Biological Process: transcription from RNA polymerase II promoter; osteoblast differentiation; regulation of apoptosis; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent. Disease: Breast Cancer

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage